Cell viability assay

2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.

CFSE (CFDA-SE) is a fluorescent dye with cell membrane permeability. CFSE irreversibly binds to intracellular proteins in living cells and is used for the detection of cell proliferation. The labeled cells fluoresce in green color with excitation wavelength of 488 nm and emission wavelength of 518 nm.

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

Thiazolyl Blue (MTT) is a colorimetric compound extensively utilized to measure cell proliferation, transitioning from a yellow color to purple formazan in living cells.

WST-1 is a cytotoxic, water-soluble tetrazolium salt that induces intracellular mitochondrial dehydrogenases to undergo NADH-dependent enzymatic reactions, releasing water-soluble methylbenzene products.WST-1 can be used to detect cell proliferation and cellular activity by assaying the uptake values, and can be used to determine superoxide dismutase activity in the WST-1 plate assay.

Evans blue (C.I. 23860) is an azo dye, inhibits L-glutamate uptake via the membrane-bound excitatory amino acid transporter (EAAT).

ATP-Red 1 is a fluorescent probe with good membrane permeability that selectively and rapidly responds to intracellular ATP concentrations in live cells.

Sulfobromophthalein disodium salt is an organic anion dye, it is used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of drugs and metabolites.

Rose Bengal sodium is a potent inhibitor of VGlut and vesicular monoamine transporter (VMAT) (Ki of 19 and 64 nM for VGlut andVMAT, respectively）

Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.

Neutral red is a vital dye, is used as an indicator and biological stain.

Resorufin sodium salt (NSC-12097 sodium salt) is a fluorescent pink dye.

Calcein Blue is a blue-fluorescent, short-term dye.

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

6-NBDG, a fluorescent glucose analogue, can be used as a fluorescent probe.6-NBDG can be used for monitoring glucose transport and uptake. 6-NBDG can be used as for detecting macrophage-rich atherosclerotic plaques.

Calcein Red AM, a red-emitting derivative of the green fluorophore calcein AM, is a cell-permeable, non-fluorescent acetoxymethyl ester compound commonly utilized to assess cell viability and cytotoxicity. following intracellular esterase cleavage, the fluorescent product is retained in live cells and exhibits characteristic excitation and emission peaks at 560 nm and 574 nm, respectively.

CytoCalcein Violet 450 is a fluorogenic dye used to assess cell viability. It is a cell-permeable dye that, upon entering cells, is cleaved by intracellular esterases. CytoCalcein Violet 450 is retained by living cells and displays excitation emission maxima of 405 450 nm, respectively.

Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation emission maxima of 525 550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.

XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator [1,2]. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis [3].

Resorufin acetate, a fluorescent dye, is used to selectively detect hydrazine [1].

Resazurin is used as an oxidation-reduction indicator in cell viability.

WST-3 is a sensitive tetrazolium reagent.

Ethidium monoazide bromide, a fluorescent dye that intercalates with DNA, selectively penetrates bacteria possessing compromised membranes. Upon photoactivation, it forms covalent bonds with DNA, enabling it to exclusively stain dead cells [1].

Calcein tetraethyl ester, a fluorescent dye, is utilized in biological studies for its ability to penetrate cellular membranes into living cells. This property renders it valuable for assessing cell viability and short-term cell labeling.