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6-CFDA

Name: 6-CFDA
Brand: TargetMol
SKU: T14057
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T14057Cas No. 3348-03-6

Alias 6-Carboxyfluorescein diacetate

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

6-CFDA

🥰Excellent

Catalog No. T14057Alias 6-Carboxyfluorescein diacetateCas No. 3348-03-6

6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$50	In Stock	In Stock
50 mg	$73	In Stock	-
1 mL x 10 mM (in DMSO)	$45	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.
In vitro	Sperm plasma membrane integrity analysis Procedure: 1. Wash cryopreserved sperm and fresh sperm 3 times by centrifugation in PBS preheated at 36°C (3000 rpm × 5 min). 2. After washing, resuspend sperm in 1 mL PBS, mix with 10 μM PI and 1 μM 6-CFDA dye, and incubate at 37°C for 15 minutes. 3. After incubation, wash sperm 3 times with PBS and obtain images under fluorescence microscope.
Synonyms	6-Carboxyfluorescein diacetate

Chemical Properties

Molecular Weight	460.39
Formula	C₂₅H₁₆O₉
Cas No.	3348-03-6
Smiles	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C22OC(=O)c3ccc(cc23)C(O)=O)c1
Relative Density.	1.57 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 97 mg/mL (210.69 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (7.17 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1721 mL	10.8604 mL	21.7207 mL	108.6036 mL
5 mM	0.4344 mL	2.1721 mL	4.3441 mL	21.7207 mL
10 mM	0.2172 mL	1.0860 mL	2.1721 mL	10.8604 mL
20 mM	0.1086 mL	0.5430 mL	1.0860 mL	5.4302 mL
50 mM	0.0434 mL	0.2172 mL	0.4344 mL	2.1721 mL
100 mM	0.0217 mL	0.1086 mL	0.2172 mL	1.0860 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc