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6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | $50 | In Stock | |
50 mg | $73 | In Stock | |
1 mL x 10 mM (in DMSO) | $45 | In Stock |
Description | 6-CFDA (6-Carboxyfluorescein diacetate) is fluorescent polyanionic probe that used to measure changes in intracellular pH (pHi) and processes such as dendrimer aggregation and absorption. |
In vitro | Sperm plasma membrane integrity analysis Procedure: 1. Wash cryopreserved sperm and fresh sperm 3 times by centrifugation in PBS preheated at 36°C (3000 rpm × 5 min). 2. After washing, resuspend sperm in 1 mL PBS, mix with 10 μM PI and 1 μM 6-CFDA dye, and incubate at 37°C for 15 minutes. 3. After incubation, wash sperm 3 times with PBS and obtain images under fluorescence microscope. |
Alias | 6-Carboxyfluorescein diacetate |
Molecular Weight | 460.39 |
Formula | C25H16O9 |
Cas No. | 3348-03-6 |
Smiles | CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C22OC(=O)c3ccc(cc23)C(O)=O)c1 |
Relative Density. | 1.57 g/cm3 (Predicted) |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | DMSO: 97 mg/mL (210.69 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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