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Calcein Red AM

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Catalog No. T36327

Calcein Red AM, a red-emitting derivative of the green fluorophore calcein AM, is a cell-permeable, non-fluorescent acetoxymethyl ester compound commonly utilized to assess cell viability and cytotoxicity. following intracellular esterase cleavage, the fluorescent product is retained in live cells and exhibits characteristic excitation and emission peaks at 560 nm and 574 nm, respectively.

Calcein Red AM

Calcein Red AM

😃Good
Catalog No. T36327
Calcein Red AM, a red-emitting derivative of the green fluorophore calcein AM, is a cell-permeable, non-fluorescent acetoxymethyl ester compound commonly utilized to assess cell viability and cytotoxicity. following intracellular esterase cleavage, the fluorescent product is retained in live cells and exhibits characteristic excitation and emission peaks at 560 nm and 574 nm, respectively.
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1 mg$96835 days
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Product Introduction

Bioactivity
Description
Calcein Red AM, a red-emitting derivative of the green fluorophore calcein AM, is a cell-permeable, non-fluorescent acetoxymethyl ester compound commonly utilized to assess cell viability and cytotoxicity. following intracellular esterase cleavage, the fluorescent product is retained in live cells and exhibits characteristic excitation and emission peaks at 560 nm and 574 nm, respectively.
In vitro
I. Preparation of Calcein Red AM Working Solution
1. Preparation of Stock Solution: DMSO can be used to prepare a 5-10 mM stock solution for future use.
2. Preparation of Working Solution: Dilute the stock solution with prewarmed pure DMEM or PBS to prepare a 1-10 μM Calcein Red AM working solution for future use.
Notes:
1) Adjust the concentration of the Calcein Red AM working solution according to actual needs and prepare it immediately before use.
2) Store unused stock solution at -80°C or -20°C, aliquot, and store in a dark place. Avoid repeated freeze-thaw cycles.
II. Cell Staining
1. Suspension Cells
1) Collect cells by centrifugation and wash twice with PBS for 5 minutes each. The cell density should be 1×10^6/mL.
2) Add 1 mL of Calcein Red AM working solution and incubate at 37°C for 30-45 minutes.
3) Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
4) Add PBS Wash the cells twice, 5 minutes each.
5) Resuspend the cells in 1 mL of pure DMEM or PBS and observe using a fluorescence microscope or flow cytometer.
2. Adherent Cells
1) Plate the adherent cells on a sterile coverslip.
2) Remove the coverslip from the culture medium and aspirate the excess medium.
3) Add 100 μL of the dye working solution, gently shake to completely cover the cells, and incubate at 37°C for 30-45 minutes.
4) Aspirate the dye working solution, wash the cells 2-3 times with culture medium, 5 minutes each, and observe using a fluorescence microscope or fluorescence microscopy reader.

Precautions
1. Please adjust the concentration of the Calcein Red AM working solution and incubation time according to your specific needs.
2. This product is for professional scientific research use only and is not intended for clinical diagnosis or other purposes.
3. For your safety and well-being, please wear a lab coat and disposable gloves when handling.
Chemical Properties
Storage & Solubility Information
Storagekeep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature.

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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