Nitric Oxide (NO) Dyes

2,3-Diaminonaphthalene is a highly selective fluorometric and colorimetric reagent for selenium detection, and it is also used for the fluorometric determination of nitrite.

DAF-2 DA (5,6-Diaminofluorescein diacetate) is a cell-permeable fluorescent probe used for detecting nitric oxide (NO), requiring use in a serum-free environment free from phenol red interference. It has an excitation wavelength of 491 nm and an emission wavelength of 513 nm, with a commonly used working concentration of 1–10 μm.

DAF-FM DA (Diaminofluorescein-FM diacetate) is a reactive dye for the detection of nitric oxide (NO) and reactive nitrogen species (RNS) levels for bioimaging (Ex/Em=495/515 nm).

Phenazine ethosulfate, a cationic dye (Ex=390 nm, Em=530 nm) and electron acceptor, is used in dye-linked enzyme assays and serves as an intermediate for detecting nitric oxide reductase (Nors) activity in the presence of ascorbic acid [1] [2] [3].

BOD-NH-NP is a fluorescent probe designed for the specific detection of endogenous nitric oxide through the eNOS enzymatic pathway [1].

HKYellow-AM (6 12-mixture) is a fluorogenic probe for the sensitive and specific detection of peroxynitrite.

DAF-FM is a diamino-fluorescein that serves as a fluorescent indicator for nitric oxide (NO) and exhibits excellent pH tolerance.

5,6-Diamino-N,N,N',N'-tetraethyl-rhodamin serves as a fluorescent indicator for NO [1].

1,2-Diaminoanthraquinone serves as a sensitive, specific, and nontoxic fluorescent probe for nitric oxide (NO), facilitating the detection of NO production in live cells and animals. It exhibits maximal absorption at approximately 540 nm and achieves a detection limit of 5 μM for NO [1] [2].

DAF-2T (Diaminofluorescein 2T) serves as a multifunctional dye utilized extensively across various domains. In the realm of biological experimentation, this dye facilitates the observation and analysis of cell structures, tracking of biomolecules, evaluation of cell functions, differentiation of cell types, detection of biomolecules, examination of tissue pathology, and monitoring of microorganisms. Its applications extend beyond fundamental scientific research into clinical diagnostics, highlighting its broad utility. Additionally, DAF-2T is employed in more traditional sectors like textile dyeing and in newer areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

RB-OPD, also known as NO-red, is a fluorescent probe that combines o-phenylenediamine (OPD) and rhodamine nitric oxide (NO). With a λex of 550 nm and a λem of 590 nm, it exhibits exceptional sensitivity and selectivity.

4,5-Diaminofluorescein serves as a fluorescent detector for nitric oxide (NO) in cells and tissues [1].