Sulfobromophthalein disodium salt is an organic anion dye, it is used in the study of a variety of membrane carriers expressed in animal tissues and involved in transport of drugs and metabolites.

I. Membrane carrier research
1. Solution preparation: Dissolve Bromosulfophthalein disodium salt in an appropriate solvent (such as saline).
2. Labeling process: By injecting a certain concentration of Bromosulfophthalein into experimental animals, researchers can track its metabolic pathways in the body and its distribution in different tissues.
3. Detection: Use a spectrophotometer or fluorescence microscope to measure the absorbance or fluorescence intensity of Sulfobromophthalein (depending on the experimental design) to analyze the function of the membrane carrier.
II. Liver function test
1. Injection: Inject Sulfobromophthalein solution into experimental animals (such as rats), and the dosage depends on the experimental needs.
2. Monitoring: By detecting changes in Bromosulfophthalein concentration in plasma, the liver's uptake and excretion capacity can be inferred, and then the liver health can be evaluated.
III. Drug metabolism research
1. Drug uptake: Inject drugs and Sulfobromophthalein into experimental animals, and use dyes as markers to track the drug transport process.
2. Monitoring: By monitoring the distribution and changes of Bromosulfophthalein in the body, the important carriers and mechanisms in the drug transport process can be studied.