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Calcein Blue

Name: Calcein Blue
Brand: TargetMol
SKU: T18933
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Synonyms:

Catalog No. T18933 Copy Product Info

Purity: 99.79%

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Calcein Blue is a coumarin-based fluorescent dye containing an iminodiacetic acid moiety that typically does not readily cross cell membranes. It can be used as a fluorescent labeling agent or as a metal ion fluorescent indicator, and is widely used in metal ion detection, cell imaging, and bioanalytical research.

Cas No. 54375-47-2

Pack Size	Price	USA Stock	Global Stock
25 mg	$40	-	In Stock
50 mg	$60	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:99.79%

Appearance:Solid

Color:White

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description

In vitro

Calcein Blue (CB) forms a Calcein Blue–Fe²⁺ complex with Fe²⁺, and its fluorescence signal is significantly reduced due to the quenching effect of Fe²⁺. When dopamine (DA) is added to this system, it competes with Fe²⁺ for coordination, forming a more stable DA–Fe²⁺ complex, thereby releasing free CB and restoring its fluorescence. Under excitation at 340 nm and emission at 440 nm, the degree of fluorescence recovery shows a good linear correlation with dopamine concentration, making it suitable for the quantitative detection of dopamine. Among the catecholamines and related compounds studied, the Calcein Blue–Fe²⁺ system exhibits good selective recognition of dopamine [2].

Chemical Properties

Molecular Weight	321.28
Formula	C₁₅H₁₅NO₇
Cas No.	54375-47-2
Smiles	Cc1cc(=O)oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
Relative Density.	1.512 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 6 mg/mL (18.68 mM), Sonication and heating are recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1125 mL	15.5627 mL	31.1255 mL	155.6275 mL
5 mM	0.6225 mL	3.1125 mL	6.2251 mL	31.1255 mL
10 mM	0.3113 mL	1.5563 mL	3.1125 mL	15.5627 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc