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WST-8

Name: WST-8
Brand: TargetMol
SKU: T4018
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4018Cas No. 193149-74-5

Alias WST8

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

WST-8

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Purity: 99.94%

Catalog No. T4018Alias WST8Cas No. 193149-74-5

WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$33	In Stock	In Stock
25 mg	$50	In Stock	In Stock
50 mg	$72	In Stock	In Stock
100 mg	$109	In Stock	In Stock
500 mg	$328	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.94%

Color:Yellow

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COA LCMS

Product Introduction

Bioactivity

Description	WST-8 is a water-soluble tetrazolium dye, and WST-8-based assays have higher sensitivity than traditional MTS-based assays.
In vitro	METHODS: A commonly used MTS-based assay was compared to a bioassay using the water-soluble tetrazolium dye WST-8 at a concentration of 7 × 10(5) cells/ml with 800 IU/ml PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. In addition, the optimized WST-8 dye-based assay was used to test the potency of various commercially available PEGylated G-CSF formulations. RESULTS The results demonstrated that the WST-8-based assay had greater sensitivity in determining the potency of PEGylated G-CSF using the NFS-60 cell line compared to the conventional MTS-based assay.[1]
Synonyms	WST8

Chemical Properties

Molecular Weight	600.47
Formula	C₂₀H₁₃N₆NaO₁₁S₂
Cas No.	193149-74-5
Smiles	[Na+].COc1cc(ccc1-[n+]1nc(nn1-c1ccc(cc1)[N+]([O-])=O)-c1ccc(cc1S([O-])(=O)=O)S([O-])(=O)=O)[N+]([O-])=O
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 50 mg/mL (83.27 mM), Sonication is recommended.

DMSO: 255 mg/mL (424.67 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (1.67 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.6654 mL	8.3268 mL	16.6536 mL	83.2681 mL
5 mM	0.3331 mL	1.6654 mL	3.3307 mL	16.6536 mL
10 mM	0.1665 mL	0.8327 mL	1.6654 mL	8.3268 mL
20 mM	0.0833 mL	0.4163 mL	0.8327 mL	4.1634 mL
50 mM	0.0333 mL	0.1665 mL	0.3331 mL	1.6654 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0167 mL	0.0833 mL	0.1665 mL	0.8327 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

WST-8WST 8Inhibitorinhibit