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Diphenyl Blue

Name: Diphenyl Blue
Brand: TargetMol
SKU: T18979
Rating: 4.5 (1 reviews)

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Catalog No. T18979Cas No. 72-57-1

Alias Direct Blue 14

Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.

Diphenyl Blue

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Purity: 96.01%

Catalog No. T18979Alias Direct Blue 14Cas No. 72-57-1

Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 g	$29	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:96.01%

Appearance:Solid

Color:Black

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.
Cell Research	I. Analysis and standardization of azo dyes 1. Dye calibration: Dissolve Diphenyl Blue in an appropriate solvent (such as water or an organic solvent) and use it as a standard solution. Compare it with other azo dyes to be tested. 2. Staining experiment: Use Diphenyl Blue to perform staining experiments and compare its color changes under different conditions (such as different pH, temperature or solvent environments). 3. Performance test: Use a colorimeter or spectrophotometer to test the absorbance and other optical properties of the dye to evaluate its staining performance. II. Dye stability and reactivity study 1. Stability test: Store Diphenyl Blue solution under different temperature, pH or light conditions, measure its color changes regularly, and evaluate the stability of the dye. 2. Reactivity study: React with other chemical substances (such as metal ions, acid-base substances, etc.) to test the chemical reactivity of Diphenyl Blue. III. Cell staining 1. Solution preparation: Prepare 0.4% trypan blue with 0.85% Nacl. For example, 400 mg of trypan blue is dissolved in 100 mL of 0.85% Nacl. 2. Cell staining: Add Diphenyl Blue solution to the cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour) to stain the cells. 3. Microscopic observation: Use a microscope to observe the staining effect, and measure the absorbance with a spectrophotometer to further analyze the intensity of the staining. IV. Quantitative analysis of dye behavior: 1. Solubility test: Dissolve different concentrations of Diphenyl Blue in various solvents, measure its solubility and observe the color change of the solution. 2. Absorbance measurement: Use a spectrophotometer to measure the absorbance of the solution at different wavelengths, draw an absorption spectrum, and analyze the optical properties of the dye. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Direct Blue 14

Chemical Properties

Molecular Weight	960.81
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Cas No.	72-57-1
Smiles	CC1=CC(C2=CC=C(/N=N/C3=C(S(=O)(O[Na])=O)C=C4C=C(S(=O)(O[Na])=O)C=C(N)C4=C3O)C(C)=C2)=CC=C1/N=N/C5=C(S(=O)(O[Na])=O)C=C6C=C(S(=O)(O[Na])=O)C=C(N)C6=C5O
Relative Density.	1.007 g/mL at 20°C

Storage & Solubility Information

Storage

keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 10 mg/mL (10.41 mM), Sonication is recommended.

DMSO: 8 mg/mL (8.33 mM), Sonication is recommended.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	1.0408 mL	5.2039 mL	10.4079 mL	52.0394 mL
5 mM	0.2082 mL	1.0408 mL	2.0816 mL	10.4079 mL

H2O

	1mg	5mg	10mg	50mg
10 mM	0.1041 mL	0.5204 mL	1.0408 mL	5.2039 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitDirect Blue-14Direct Blue14Diphenyl Blue