Diphenyl Blue (Direct Blue 14) (Direct Blue 14) is used as a dye for a group of azo dye.

I. Analysis and standardization of azo dyes
1. Dye calibration: Dissolve Diphenyl Blue in an appropriate solvent (such as water or an organic solvent) and use it as a standard solution. Compare it with other azo dyes to be tested.
2. Staining experiment: Use Diphenyl Blue to perform staining experiments and compare its color changes under different conditions (such as different pH, temperature or solvent environments).
3. Performance test: Use a colorimeter or spectrophotometer to test the absorbance and other optical properties of the dye to evaluate its staining performance.
II. Dye stability and reactivity study
1. Stability test: Store Diphenyl Blue solution under different temperature, pH or light conditions, measure its color changes regularly, and evaluate the stability of the dye.
2. Reactivity study: React with other chemical substances (such as metal ions, acid-base substances, etc.) to test the chemical reactivity of Diphenyl Blue.
III. Cell staining
1. Solution preparation: Prepare 0.4% trypan blue with 0.85% Nacl. For example, 400 mg of trypan blue is dissolved in 100 mL of 0.85% Nacl.
2. Cell staining: Add Diphenyl Blue solution to the cell culture medium and incubate for a certain period of time (usually 30 minutes to 1 hour) to stain the cells.
3. Microscopic observation: Use a microscope to observe the staining effect, and measure the absorbance with a spectrophotometer to further analyze the intensity of the staining.
IV. Quantitative analysis of dye behavior:
1. Solubility test: Dissolve different concentrations of Diphenyl Blue in various solvents, measure its solubility and observe the color change of the solution.
2. Absorbance measurement: Use a spectrophotometer to measure the absorbance of the solution at different wavelengths, draw an absorption spectrum, and analyze the optical properties of the dye.