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Fluo-3AM (1 mg/ml solution in acetonitrile)
(Synonyms: Fluo-3-pentaacetoxymethyl ester) Copy Product Info
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Synonyms: Fluo-3-pentaacetoxymethyl ester
Catalog No. T18989 Copy Product Info
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Fluo-3AM is a cell-permeable calcium ion fluorescent probe precursor. Its AM ester form crosses cell membranes and is hydrolyzed by intracellular esterases to generate Fluo-3, which specifically binds Ca²⁺ and enhances fluorescence signal. Fluo-3AM is widely used to monitor intracellular calcium concentration changes and calcium signal transduction processes.
Cas No. 121714-22-5
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 500 µg | $156 | - | In Stock | |
| 1 mg | $263 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Color:Transparent
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Fluo-3AM is a cell-permeable calcium ion fluorescent probe precursor. Its AM ester form crosses cell membranes and is hydrolyzed by intracellular esterases to generate Fluo-3, which specifically binds Ca²⁺ and enhances fluorescence signal. Fluo-3AM is widely used to monitor intracellular calcium concentration changes and calcium signal transduction processes. |
| Synonyms | Fluo-3-pentaacetoxymethyl ester |
| Molecular Weight | 1129.85 |
| Formula | C51H50Cl2N2O23 |
| Cas No. | 121714-22-5 |
| Smiles | O=C(OCOC(=O)CN(C=1C=CC(=CC1OCCOC2=CC(=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)C)C3=C4C=C(Cl)C(=O)C=C4OC=5C=C(OCOC(=O)C)C(Cl)=CC53)CC(=O)OCOC(=O)C)C |
| Relative Density. | 1.49 g/cm3 (Predicted) |
| Storage | Keep away from direct sunlight Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||
| Solubility Information | DMSO: 10 mg/mL (8.85 mM), Sonication is recommended. | |||||||||||||||
Solution Preparation Table | ||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Fluo-3AM (1 mg/ml solution in acetonitrile) chemical structure | Fluo-3AM (1 mg/ml solution in acetonitrile) formula | Fluo-3AM (1 mg/ml solution in acetonitrile) molecular weight
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