Resorufin sodium salt (NSC-12097 sodium salt) is a fluorescent pink dye.

1. For the detection of ROS and RNS
1. Solution preparation: Dissolve Resorufin sodium salt in an appropriate solvent (such as deionized water or PBS), usually preparing a working solution with a concentration of 0.1-10 μM.
2. Treatment: Add the solution to the cell culture medium or tissue section and incubate for a certain period of time (usually 15-120 minutes) to allow the dye to enter the cell and react with ROS/RNS.
3. Fluorescence detection: Fluorescence detection is performed by fluorescence microscopy, fluorescence spectrophotometer or flow cytometer. The excitation wavelength of Resorufin is 540 nm and the emission wavelength is 590 nm.
4. Data analysis: The concentration of ROS/RNS is proportional to the intensity of the fluorescence signal, and the change of ROS/RNS can be quantitatively evaluated by the change of the fluorescence signal intensity.
2. For the detection of biomarkers and analytes
1. Labeling reaction: Resorufin sodium salt reacts with the target biomolecule or probe to bind to the analyte.
2. Detection and analysis: Use a fluorescence microscope or spectrophotometer to perform quantitative analysis based on the change in fluorescence signal intensity after the reactant binds to the dye.
III. Used for cell activity and function research
1. Cell treatment: Add Resorufin sodium salt to the cell culture medium and observe the fluorescence changes after incubation for a certain period of time.
2. Cell viability assessment: Evaluate the increase or decrease in ROS levels in cells through changes in fluorescence intensity, and then infer the physiological state of cells.
IV. Application in animal models
1. Animal treatment: Inject or gavage Resorufin sodium salt into animals, or use it in in vitro tissue samples of animals.
2. Fluorescence imaging: Evaluate the changes of ROS/RNS under different experimental conditions through in vivo or in vitro imaging techniques (such as fluorescence imaging or fluorescence detection of tissue sections).