Fluorescein

FITC is a green fluorescein derivative. FITC is often used to label biological macromolecules such as antibodies and proteins. FITC can also be used to label nucleic acids. FITC can be used as a fluorescent probe to detect cell viability, cell apoptosis, etc. FITC showed a maximum excitation wavelength of 494 nm and yellow-green fluorescence at a maximum emission wavelength of 520 nm.

GLF16 FA is a fluorescent SBB analogue for rapid detection of senescent cells using flow cytometry and live cell sorting.

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

Fluorescein (Solvent Yellow 94) is a phthalic indicator dye, use as a synthetic fluorescent tracer.

5-CFDA (5-Carboxyfluorescein diacetate)(5-Carboxyfluorescein diacetate) is membrane-permeant. It can be loaded into cells via incubation and hydrolyzed by intracellular esterases to 5-carboxyfluorescein that is used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.

5-FAM (5-Carboxyfluorescein) (5-Carboxyfluorescein) contains a carboxylic acid, which can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its excellent fluorescence quantum yield, relatively high absorptivity, and good water solubility, it also has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.

5(6)-FITC (Fluorescein isothiocyanate), or Fluorescein 5(6)-isothiocyanate, is a versatile amine-reactive derivative of fluorescein dye. It serves as an effective label for antibodies and other probes, finding utility in fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as ELISA and Western blotting.

Fmoc-Lys(5-FITC)-OH is a fluorescent probe used to detect amine labelling of peptides or proteins and can serve as a building block for organic synthesis.

ACE TFA is a non-GFP class fluorescent dye. It binds with fluorescent RNA aptamers, facilitating the study of RNA visualization in living cells.

6-DTAF hydrochloride, a fluorescein derivative, is utilized for labeling peptides and nucleotides [1].

Europium W1024 isothiocyanate (ITC) is a lanthanide-based fluorophore characterized by its long emission lifetime and significant Stokes shift, featuring excitation and emission maxima at 340 and 613 nm, respectively. This compound includes a reactive isothiocyanate handle that engages with primary amino groups in alkaline conditions. It maintains stability under high temperatures, in the face of elevated EDTA concentrations, or at low pH levels, though it falters when exposed to manganese. Commonly, Europium W1024 ITC serves as a donor fluorophore in time-resolved fluorescence resonance energy transfer (TR-FRET) applications, offering the advantage of a prolonged measurement window for observing acceptor fluorescence and minimizing autofluorescence interference through its extended emission lifetime.

5-DTAF, an isomeric derivative of fluorescein, reacts directly with polysaccharides and alcohols in aqueous solutions at pH > 9. Its emission profile peaks at a wavelength of 518 nm following excitation at 488 nm [1].

Sulfane Sulfur Probe 4 (SSP4) serves as a fluorescent probe targeting sulfane sulfur species, encompassing hydrogen sulfide-derived polysulfides, hydropersulfides, and persulfides generated endogenously. Upon interacting with these species, SSP4 undergoes a nucleophilic attack, leading to fluorescein production, characterized by 494 515 nm excitation emission peaks, along with the generation of two 1,2-benzodithiol-3-one molecules. This compound has been instrumental in localizing H2S3 within primary mouse neurons and identifying polysulfides in L. japonicus seedling roots.

5-FAM-LPETGG is a fluorescent peptide that combines the sortase recognition sequence LPETGG with the fluorescent label 5-carboxyfluorescein (5-FAM), characterized by excitation emission maxima at 492 518 nm. It serves for site-specific labeling of target proteins through sortase-catalyzed transpeptidation.

5MP-Fluorescein (compound 3e), a fluorescein dye derived from 5-Methylene pyrrolone (5MP), exemplifies a bioconjugation tool that is both highly thiol-specific and can be removed without a trace [1].

5(6)-Carboxyeosin-diacetate serves as a versatile dye with a broad spectrum of applications in both traditional and emerging fields. This compound is vital in biological experiments for observing and analyzing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its utility extends beyond basic scientific research to encompass clinical diagnostics. Additionally, dyes like 5(6)-Carboxyeosin-diacetate are essential in industries such as textile dyeing, functional textile processing, food coloring, and the development of dye-sensitized solar cells.

Fluorescein diacetate 5-maleimide, a colorimetric probe, is synthesized through the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.

Fluorescein diacetate 6-isothiocyanate, a colorimetric probe, is formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.

6-Fluorescein Isothiocyanate (6-FITC) is a fluorescein derivative widely employed in applications such as flow cytometry.

5(6)-Carboxy-eosin, a multifunctional dye, serves as a crucial tool across various scientific, clinical, and industrial applications. In biological experiments, this dye enables researchers to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish between cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its utility extends beyond basic research to encompass a broad spectrum of diagnostics. Additionally, 5(6)-Carboxy-eosin finds extensive use in traditional sectors like textile dyeing and in innovative areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

5-Bromomethyl-fluorescein is a versatile dye utilized in various biological experiments. Dyes are crucial for observing cell structures, tracking biomolecules, evaluating cellular functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Their applications extend from fundamental research to clinical diagnostics. Additionally, dyes are employed in traditional areas like textile dyeing and in new areas such as functional textile processing, food pigments, and dye-sensitized solar cells.

Fluorescein O,O′-diacrylate (Diacryloyloxyfluoresin) is a fluorescent compound known for its excellent biocompatibility and cellular labeling capabilities. It is frequently utilized in biological imaging and cell tracking studies to effectively mark cells and monitor their dynamic changes within an organism. Fluorescein O,O′-diacrylate is also applied in compound delivery systems to enhance the precision of compound localization and its inhibitory effects. Additionally, it is used in the preparation of polymer materials to improve their optical properties and biological interactions.

Fluorescent brightener 210 is a versatile dye that plays a crucial role in biological experiments by aiding in the observation and analysis of cell structures, biomolecule tracking, and cell function evaluation. It helps distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. The applications of this dye extend from fundamental scientific research to a broad spectrum of clinical diagnostics and are widely utilized in traditional areas like textile dyeing, as well as in innovative sectors such as functional textile processing, food pigments, and dye-sensitized solar cells.

Fluorescein-diacetate-5-isothiocyanate is a colorimetric probe synthesized through the reaction between adipic acid dihydrazide and fluorescein isothiocyanate.