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6-FAM SE

Name: 6-FAM SE
Brand: TargetMol
SKU: T18901
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18901Cas No. 92557-81-8

Alias 6-Carboxyfluorescein N-succinimidyl ester

6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein. 6-FAM SE is mainly used for labeling nucleotides and sequencing nucleic acids.

6-FAM SE

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Purity: 98.23%

Catalog No. T18901Alias 6-Carboxyfluorescein N-succinimidyl esterCas No. 92557-81-8

6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein. 6-FAM SE is mainly used for labeling nucleotides and sequencing nucleic acids.

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$43	In Stock	In Stock
25 mg	$78	In Stock	In Stock
50 mg	$143	In Stock	In Stock
100 mg	$238	In Stock	In Stock
200 mg	$353	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.23%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Bioactivity

Description	6-FAM SE (6-Carboxyfluorescein N-succinimidyl ester) is another isomer of carboxyfluorescein. 6-FAM SE is mainly used for labeling nucleotides and sequencing nucleic acids.
In vitro	I. Nucleic acid sequencing 1. Labeling nucleotides: 6-FAM SE reacts with the 5' end of the nucleotide or oligonucleotide, and the succinic anhydride group reacts with the primary amine group in the nucleic acid to form a covalently bound fluorescent dye. 2. Fluorescence detection: During the sequencing process, the labeled nucleotides will emit fluorescence (excitation wavelength: 495 nm, emission wavelength: 520 nm), and the automatic sequencer can detect the fluorescence signal to determine the base sequence. II. Gene expression analysis 1. Labeling probes: 6-FAM is used as probes in qPCR experiments, which are designed to bind to specific regions of the target gene. 2. Quantification: The fluorescence signal generated during the amplification process is proportional to the number of target genes, so that gene expression can be quantitatively analyzed. III. Molecular biology detection: 1. Probe preparation: 6-FAM is labeled on a nucleic acid probe, which will hybridize with the target sequence. b. Detection: The fluorescence signal emitted by the 6-FAM-labeled probe is detected by fluorescence microscopy. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	6-Carboxyfluorescein N-succinimidyl ester

Chemical Properties

Molecular Weight	473.39
Formula	C₂₅H₁₅NO₉
Cas No.	92557-81-8
Smiles	Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3ccc(cc23)C(=O)ON2C(=O)CCC2=O)c1
Relative Density.	1.74g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 45 mg/mL (95.06 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (4.22 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1124 mL	10.5621 mL	21.1242 mL	105.6212 mL
5 mM	0.4225 mL	2.1124 mL	4.2248 mL	21.1242 mL
10 mM	0.2112 mL	1.0562 mL	2.1124 mL	10.5621 mL
20 mM	0.1056 mL	0.5281 mL	1.0562 mL	5.2811 mL
50 mM	0.0422 mL	0.2112 mL	0.4225 mL	2.1124 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc