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D-Luciferin

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Catalog No. T3313Cas No. 2591-17-5
Alias Firefly luciferin, D-(-)-Luciferin

D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays.

D-Luciferin
Other Forms of D-Luciferin:

D-Luciferin

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Purity: 99.83%
Catalog No. T3313Alias Firefly luciferin, D-(-)-LuciferinCas No. 2591-17-5
D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays.
Pack SizePriceAvailabilityQuantity
10 mg$45 In Stock
50 mg$82 In Stock
100 mg$119 In Stock
200 mg$178 In Stock
500 mg$298 Backorder
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Purity:99.83%
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COA HNMR HPLC
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Product Introduction

Bioactivity
Description
D-Luciferin (D-(-)-Luciferin) is a luciferase (Luc) substrate that catalyzes the production of typical yellow-green light (560 nm) by bioluminescent insects. D-Luciferin can be used in luciferase assays.
In vitro
D-luciferin reacts with luciferase, ATP and oxygen with light emission, and the light is detected by a sensitive photographic film, thereby permitting the visualization of the alkaline phosphatase-conjugated antibodies. [1]
In vivo
In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. [2]
AliasFirefly luciferin, D-(-)-Luciferin
Chemical Properties
Molecular Weight280.32
FormulaC11H8N2O3S2
Cas No.2591-17-5
SmilesOC(=O)[C@H]1CS\C(N1)=c1\nc2C=CC(=O)C=c2s1
Relative Density.1.81g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: 1 mg/mL (3.57 mM), Sonication is recommended.
DMSO: 55 mg/mL (196.2 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+90% Saline: 5.5 mg/mL (19.62 mM), Solution.
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5.5 mg/mL (19.62 mM), Solution.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM3.5674 mL17.8368 mL35.6735 mL178.3676 mL
DMSO
1mg5mg10mg50mg
5 mM0.7135 mL3.5674 mL7.1347 mL35.6735 mL
10 mM0.3567 mL1.7837 mL3.5674 mL17.8368 mL
20 mM0.1784 mL0.8918 mL1.7837 mL8.9184 mL
50 mM0.0713 mL0.3567 mL0.7135 mL3.5674 mL
100 mM0.0357 mL0.1784 mL0.3567 mL1.7837 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Keywords

InhibitorinhibitD-LuciferinDLuciferinD LuciferinBeetle Luciferin
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