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Fluorescein diphosphate tetraammonium

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Catalog No. T20421Cas No. 217305-49-2
Alias Fluorescein diphosphate Tetraammonium salt, FDP

Fluorescein diphosphate tetraammonium is a fluorescent dye and a fluorescent phosphatase substrate that is hydrolyzed into highly fluorescent fluorescein after interaction with phosphatases.

Fluorescein diphosphate tetraammonium

Fluorescein diphosphate tetraammonium

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Purity: 95.12%
Catalog No. T20421Alias Fluorescein diphosphate Tetraammonium salt, FDPCas No. 217305-49-2
Fluorescein diphosphate tetraammonium is a fluorescent dye and a fluorescent phosphatase substrate that is hydrolyzed into highly fluorescent fluorescein after interaction with phosphatases.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 mg$112In StockIn Stock
5 mg$269In StockIn Stock
10 mg$393In StockIn Stock
25 mg$672In StockIn Stock
50 mg$956-In Stock
100 mg$1,290-In Stock
200 mg$1,750-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:95.12%
Appearance:Solid
Color:Orange
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Product Introduction

Bioactivity
Description
Fluorescein diphosphate tetraammonium is a fluorescent dye and a fluorescent phosphatase substrate that is hydrolyzed into highly fluorescent fluorescein after interaction with phosphatases.
In vitro
50 μM Fluorescein diphosphate tetraammonium at room temperature can be used in vitro phosphatase assays as the substrate. [1]
The reaction of Fluorescein diphosphate tetra-ammonium sal with ALP liberates the green fluorescent dye, fluorescein. [2]
SynonymsFluorescein diphosphate Tetraammonium salt, FDP
Chemical Properties
Molecular Weight560.39
FormulaC20H26N4O11P2
Cas No.217305-49-2
SmilesO=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC(OP([O-])([O-])=O)=CC=C52)OP([O-])([O-])=O.[NH4+].[NH4+].[NH4+].[NH4+]
Relative Density.1.31g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 1 mg/mL (1.78 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.7845 mL8.9224 mL17.8447 mL89.2236 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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