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Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).
Pack Size | Price | Availability | Quantity |
---|---|---|---|
5 mg | $32 | In Stock | |
10 mg | $52 | In Stock | |
25 mg | $101 | In Stock | |
50 mg | $150 | In Stock | |
100 mg | $218 | In Stock | |
1 mL x 10 mM (in DMSO) | $44 | In Stock |
Description | Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm). |
Cell Research | Instructions I. Solution preparation 1. Stock solution: Prepare 1 mM Fluorescein-5-maleimide stock solution using deionized PBS (d-PBS). 2. Working solution: Dilute the stock solution to an appropriate working concentration, usually 0.1–1 μM, according to experimental needs. II. Operation steps 1. Isolate PBMC from venous blood using Ficoll-Paque density gradient centrifugation and wash with PBS. 2. Label the washed PBMC with anti-human CD3 antibody (10 million cells per 100 μL suspension). 3. Stain with 0.1 μM Fluorescein-5-maleimide and analyze by flow cytometry. Notes: 1. Fluorescein-5-maleimide is light-sensitive and should be stored and handled away from light. 2. Storage conditions: -5°C to -30°C, away from light. 3. Before use, make sure the solution is free of particles and avoid the solution from condensing into clumps due to moisture. |
Alias | N-(5-Fluoresceinyl)maleimide |
Molecular Weight | 427.36 |
Formula | C24H13NO7 |
Cas No. | 75350-46-8 |
Smiles | Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)N2C(=O)C=CC2=O)c1 |
Relative Density. | 1.73 g/cm3 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
Solubility Information | H2O: < 0.1 mg/mL (insoluble) DMSO: 80 mg/mL (187.20 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
DMSO
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