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Fluorescein-5-maleimide
🥰Excellent
Catalog No. T18991Cas No. 75350-46-8
Alias N-(5-Fluoresceinyl)maleimide
Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).
Fluorescein-5-maleimide
🥰Excellent
Purity: 97.42%
Catalog No. T18991Alias N-(5-Fluoresceinyl)maleimideCas No. 75350-46-8
Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 mg | $32 | In Stock | In Stock | |
| 10 mg | $52 | In Stock | In Stock | |
| 25 mg | $101 | In Stock | In Stock | |
| 50 mg | $150 | In Stock | In Stock | |
| 100 mg | $218 | In Stock | In Stock | |
| 1 mL x 10 mM (in DMSO) | $44 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:97.42%
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm). |
| Cell Research | Instructions I. Solution preparation 1. Stock solution: Prepare 1 mM Fluorescein-5-maleimide stock solution using deionized PBS (d-PBS). 2. Working solution: Dilute the stock solution to an appropriate working concentration, usually 0.1–1 μM, according to experimental needs. II. Operation steps 1. Isolate PBMC from venous blood using Ficoll-Paque density gradient centrifugation and wash with PBS. 2. Label the washed PBMC with anti-human CD3 antibody (10 million cells per 100 μL suspension). 3. Stain with 0.1 μM Fluorescein-5-maleimide and analyze by flow cytometry. Notes: 1. Fluorescein-5-maleimide is light-sensitive and should be stored and handled away from light. 2. Storage conditions: -5°C to -30°C, away from light. 3. Before use, make sure the solution is free of particles and avoid the solution from condensing into clumps due to moisture. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | N-(5-Fluoresceinyl)maleimide |
| Molecular Weight | 427.36 |
| Formula | C24H13NO7 |
| Cas No. | 75350-46-8 |
| Smiles | Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)N2C(=O)C=CC2=O)c1 |
| Relative Density. | 1.73 g/cm3 |
| Color | Orange |
| Appearance | Solid |
| Storage | keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: < 0.1 mg/mL (insoluble) DMSO: 131.25 mg/mL (307.12 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSO (stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyThe above example illustrates how to use "In Vivo Formulation Calculator" and does not represent a recommended formulation for any specific compound. Please select an appropriate dissolution method based on your experimental animals and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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