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Fluorescein-5-maleimide

Name: Fluorescein-5-maleimide
Brand: TargetMol
SKU: T18991
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T18991Cas No. 75350-46-8

Alias N-(5-Fluoresceinyl)maleimide

Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).

Fluorescein-5-maleimide

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Purity: 97.42%

Catalog No. T18991Alias N-(5-Fluoresceinyl)maleimideCas No. 75350-46-8

Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).

Pack Size	Price	Availability
5 mg	$32	In Stock
10 mg	$52	In Stock
25 mg	$101	In Stock
50 mg	$150	In Stock
100 mg	$218	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock

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Batch Information

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Purity:97.42%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	Fluorescein-5-maleimide (N-(5-Fluoresceinyl)maleimide) is a fluorescent thiol-reactive dye (excitation: 494 nm, emission: 519 nm).
Cell Research	Instructions I. Solution preparation 1. Stock solution: Prepare 1 mM Fluorescein-5-maleimide stock solution using deionized PBS (d-PBS). 2. Working solution: Dilute the stock solution to an appropriate working concentration, usually 0.1–1 μM, according to experimental needs. II. Operation steps 1. Isolate PBMC from venous blood using Ficoll-Paque density gradient centrifugation and wash with PBS. 2. Label the washed PBMC with anti-human CD3 antibody (10 million cells per 100 μL suspension). 3. Stain with 0.1 μM Fluorescein-5-maleimide and analyze by flow cytometry. Notes: 1. Fluorescein-5-maleimide is light-sensitive and should be stored and handled away from light. 2. Storage conditions: -5°C to -30°C, away from light. 3. Before use, make sure the solution is free of particles and avoid the solution from condensing into clumps due to moisture.
Synonyms	N-(5-Fluoresceinyl)maleimide

Chemical Properties

Molecular Weight	427.36
Formula	C₂₄H₁₃NO₇
Cas No.	75350-46-8
Smiles	Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)N2C(=O)C=CC2=O)c1
Relative Density.	1.73 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 0.1 mg/mL (insoluble)

DMSO: 80 mg/mL (187.20 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3399 mL	11.6997 mL	23.3995 mL	116.9974 mL
5 mM	0.4680 mL	2.3399 mL	4.6799 mL	23.3995 mL
10 mM	0.2340 mL	1.1700 mL	2.3399 mL	11.6997 mL
20 mM	0.1170 mL	0.5850 mL	1.1700 mL	5.8499 mL
50 mM	0.0468 mL	0.2340 mL	0.4680 mL	2.3399 mL
100 mM	0.0234 mL	0.1170 mL	0.2340 mL	1.1700 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

InhibitorinhibitFluorescein-5-maleimideFluorescein5maleimideFluorescein 5 maleimide