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6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 mg | $42 | In Stock | |
100 mg | $76 | In Stock | |
500 mg | $197 | In Stock | |
1 mL x 10 mM (in DMSO) | $46 | In Stock |
Description | 6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation. |
In vitro | 6-FAM labeled oligonucleotide probes for fluorescence in situ hybridization (FISH) I. Solution preparation: 1. Preparation of stock solution: Dissolve 6-FAM in DMSO or ethanol to prepare a 1-10 mM stock solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting) 2. Preparation of working solution: Dilute the 6-FAM stock solution with PBS or other suitable buffer, usually 1-10 µM; (Select the appropriate working solution concentration according to experimental requirements, and prepare it for immediate use) II. Operation steps: 1. Sample preparation: Prepare chromosome samples of chicken embryonic cells, including colchicine treatment, hypotonic swelling and methanol-glacial acetic acid fixation. 2. Pretreatment: Chromosomes are treated with RNase A, pepsin and formaldehyde. 3. Hybridization: 6-FAM labeled oligonucleotides were dissolved as probes in hybridization buffer (50% formamide, 2×SSC, 10% dextran sulfate) containing E. coli tRNA at a concentration of 20 ng/μL. After co-denaturation of DNA and probe, hybridization was performed overnight at room temperature in a humidified box; 4. Washing and counterstaining: After hybridization, the slides were washed three times in 2×SSC, dehydrated in a series of 70%-96% ethanol, and sealed with an antifade containing DAP I after drying; 5. Observation and analysis: The samples were observed using a Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters, and the chromosome color images were captured and processed using QFISH software. |
Alias | 6-Carboxyfluorescein |
Molecular Weight | 376.32 |
Formula | C21H12O7 |
Cas No. | 3301-79-9 |
Smiles | OC(=O)c1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31 |
Relative Density. | 1.73 g/cm3 |
Storage | keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature. | ||||||||||||||||||||||||||||||
Solubility Information | DMSO: 35 mg/mL (93.01 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
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