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6-FAM

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Catalog No. T18902Cas No. 3301-79-9
Alias 6-Carboxyfluorescein

6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.

6-FAM

6-FAM

🥰Excellent
Purity: 98.73%
Catalog No. T18902Alias 6-CarboxyfluoresceinCas No. 3301-79-9
6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.
Pack SizePriceAvailabilityQuantity
25 mg$42In Stock
100 mg$76In Stock
500 mg$197In Stock
1 mL x 10 mM (in DMSO)$46In Stock
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Purity:98.73%
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Product Introduction

Bioactivity
Description
6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.
In vitro
6-FAM labeled oligonucleotide probes for fluorescence in situ hybridization (FISH)
I. Solution preparation:
1. Preparation of stock solution: Dissolve 6-FAM in DMSO or ethanol to prepare a 1-10 mM stock solution; (it is recommended to store at -20 ℃ or -80 ℃ in the dark after aliquoting)
2. Preparation of working solution: Dilute the 6-FAM stock solution with PBS or other suitable buffer, usually 1-10 µM; (Select the appropriate working solution concentration according to experimental requirements, and prepare it for immediate use)
II. Operation steps:
1. Sample preparation: Prepare chromosome samples of chicken embryonic cells, including colchicine treatment, hypotonic swelling and methanol-glacial acetic acid fixation.
2. Pretreatment: Chromosomes are treated with RNase A, pepsin and formaldehyde.
3. Hybridization: 6-FAM labeled oligonucleotides were dissolved as probes in hybridization buffer (50% formamide, 2×SSC, 10% dextran sulfate) containing E. coli tRNA at a concentration of 20 ng/μL. After co-denaturation of DNA and probe, hybridization was performed overnight at room temperature in a humidified box;
4. Washing and counterstaining: After hybridization, the slides were washed three times in 2×SSC, dehydrated in a series of 70%-96% ethanol, and sealed with an antifade containing DAP I after drying;
5. Observation and analysis: The samples were observed using a Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters, and the chromosome color images were captured and processed using QFISH software.
Alias6-Carboxyfluorescein
Chemical Properties
Molecular Weight376.32
FormulaC21H12O7
Cas No.3301-79-9
SmilesOC(=O)c1ccc2C(=O)OC3(c2c1)c1ccc(O)cc1Oc1cc(O)ccc31
Relative Density.1.73 g/cm3
Storage & Solubility Information
Storagekeep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice./Shipping at ambient temperature.
Solubility Information
DMSO: 35 mg/mL (93.01 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.6573 mL13.2866 mL26.5731 mL132.8656 mL
5 mM0.5315 mL2.6573 mL5.3146 mL26.5731 mL
10 mM0.2657 mL1.3287 mL2.6573 mL13.2866 mL
20 mM0.1329 mL0.6643 mL1.3287 mL6.6433 mL
50 mM0.0531 mL0.2657 mL0.5315 mL2.6573 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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