5-FAM SE is a single isomer widely employed in the preparation of various small fluorescent molecules. It is a highly popular green fluorescent reagent used for labeling peptides, proteins, and nucleotides [5-FAM SE].

Instructions:
1. Preparation of 5-FAM SE solution: Dissolve 5-FAM SE in an appropriate solvent, such as DMSO or DMF, to prepare a high concentration stock solution.
Note: Avoid vigorous stirring or shaking during dissolution to prevent fluorescence quenching.
2. Coupling reaction:
1) Mix the 5-FAM SE solution with the target biomolecule and use an appropriate buffer (usually pH 8-9.5) to promote the reaction of the succinimidyl ester group with the amine group on the biomolecule.
2) Allow the reaction mixture to incubate for an appropriate amount of time to ensure that the coupling is complete.
3. Purification:
1) After the reaction, the labeled biomolecule needs to be purified to remove unreacted 5-FAM SE and byproducts.
2) Common purification methods include dialysis, gel filtration, or chromatography, depending on the nature of the biomolecule.
4. Storage: Store the labeled biomolecule under recommended conditions, usually at -20°C, and protect from light to maintain its stability and fluorescence intensity.