TO-PRO 1

TO-PRO 1, a DNA-binding fluorochrome, is attached to the surface of Feraheme (FH) nanoparticles (NP) to create a fluorochrome-functionalized NP. It binds DNA via intercalation and serves as a crucial fluorochrome for identifying necrotic cells [1]. For storage, it is essential to protect it from light.

Z-Gly-Pro-Arg-AMC hydrochloride is a synthetic peptide that is often used as a trypsin and histone K fluorescent substrate to measure enzyme activity, which fluoresces when the enzyme is cleaved from the peptide.

Z-Gly-Pro-Arg-4MβNA is a thrombin substrate that, when cleaved by thrombin, releases 4-methoxy-2-naphthylamine (4MβNA). The liberated 4MβNA reacts with 5-nitrosalicylaldehyde, forming an insoluble yellow fluorescent product, which indicates the site of thrombin activity [1].

Ala-Phe-Pro-pNA, a chromogenic substrate of tripeptidyl peptidase, can be utilized to assess tripeptidyl peptidase activity [1].

Sar-Pro-Arg-pNA, a chromogenic substrate of α-thrombin, can be used to test α-thrombin activity [1].

Suc-Ala-Glu-Pro-Phe-pNA is a chromogenic substrate for PPIase (peptidyl-prolyl cis-trans isomerase), which releases pNA with an absorbance of 390 nm upon binding to PPIase, enabling quantitative detection of Pin1 enzyme activity.