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Flag Tag Nanobody Immunomagnetic Beads

Name: Flag Tag Nanobody Immunomagnetic Beads
Brand: TargetMol
SKU: C0155
Price: 25 EUR
Availability: InStock
Rating: 4.5 (4 reviews)

Catalog No. C0155

TargetMol's Flag Tag Nanobody Immunomagnetic Beads specifically bind to proteins tagged with the DYKDDDDK (Flag) sequence. They can be used for immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is suitable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and more.
Nanobodies are variable domain fragments (VHH) derived from the heavy-chain-only antibodies naturally found in camelids (e.g., camels, llamas, alpacas). They are currently the smallest known functional antibody unit, typically only about 12–15 kDa in size—approximately one-tenth the size of a conventional IgG antibody. Compared to traditional IgG antibodies, nanobodies are smaller, have higher affinity, lack interference from light and heavy chains, and can access epitopes more closely, reducing steric hindrance. Additionally, they exhibit greater binding stability, require milder elution conditions, and better preserve the biological activity of the sample.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Pack Size	Price	Availability
500 μL	$350	7-10 days
1 mL	$590	7-10 days
5 mL	$2,845	7-10 days

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Product Information

Flag Tag Immunomagnetic Beads	Features
Bead Size	2 μm
Binding Capacity	≥ 1.5 mg Flag Protein/mL Beads
Storage Solution	20 mM PBS, 5% BSA
Application	IP , Co-IP, ChIP、RIP

Features

1.No Antibody Chain Interference: Whether using denaturing or non-denaturing elution, the immunoprecipitated products are free from antibody heavy or light chains, facilitating downstream Western blot analysis.

2.Ultra-High Affinity: Exhibits nanomolar-level binding affinity, suitable for low-expression or hard-to-transfect cell samples.

3.Strong Binding Capacity: Directional coupling technology allows 10 μL of magnetic beads to bind approximately 15 μg of target recombinant protein.

4.High Specificity: Validated in over 10 blank cell lines with minimal non-specific adsorption.

5.Flexible Tag Recognition: Specifically binds to both 1× and 3× Flag tags at either the N- or C-terminus of the bait protein, offering broad compatibility.

6.Heat Resistance: Tested at 45℃ for thermal stability, ensuring reliable performance during transportation and room-temperature storage.

Prepare Reagents

Reagent	Formulation
Washing Buffer (1×)	TBST: 50 mM Tris-HCl，150 mM NaCl, 0.1%(v/v) Tween-20，pH7.4
Flag Peptide Elution Buffer	PBS，1 mg/mL 3× Flag peptide (TP1274)，pH 7.4
Acidity Elution Buffer	0.1M Glycine，0.1% (v/v) Tween-20，pH2.5
Neutralization Buffer	1 M Tris-HCl, pH 9.0

Instructions

Preparation of Cell Lysates

Select an appropriate lysis buffer to lyse cell samples and obtain cell lysates. Place on ice or store at -20℃ for long-term use.

Pretreatment of Magnetic Beads

1)Vortex for 1 min to resuspend the immunomagnetic beads. Take 20-30 µL of suspension and place it in a 1.5 mL EP tube.

2)Add 500 μL of Washing Buffer to the EP tube and gently invert several times to resuspend the beads. Keep the EP tube in a magnetic separator and stand for 1 min for magnetic separation. Finally, remove the supernatant and then take off the EP tube. Repeat the washing steps twice.

Immunoprecipitation

(1)Add 500 μL of prepared cell lysates to the EP tube. Place it on a rotating mixer and rotate at 37℃ for 30 min. For weak binding, incubate at room temperature for 1 hour or overnight at 4℃.

(2)After incubation, perform magnetic separation, then remove or save the supernatant for further analysis.

(3)Add 500 μL of Washing Buffer to the EP tube. Perform magnetic separation. Finally, remove the supernatant and then take off the EP tube. Repeat the washing steps 3 times.

Elution of Target Proteins

(1)Denaturing Elution: Suitable for SDS-PAGE detection. Add 100 μL of SDS-PAGE Loading Buffer to the EP tube. Mix well and heat at 95℃ for 5 min. Perform magnetic separation or centrifugation (room temperature, 13000 g, 10 min) to collect the supernatant.

(2)Neutral Elution: Add 50 μL of Flag Peptide Elution Buffer to the EP tube. Incubate on a rotating mixer at 37℃ for 5-10 min (longer incubation time when below 37℃). Then perform magnetic separation or centrifugation to collect the supernatant.

(3)Acidity Elution: Add 100 μL of Acidity Elution Buffer to the EP tube. Incubate on a rotating mixer at 37℃ for 5-10 min. Perform magnetic separation or centrifugation to collect the supernatant. To adjust the pH of acidic elution buffer to neutral, add 50 μL of Neutralization Buffer to 100 μL elution.

Note: Nanobody-conjugated magnetic beads do not have heavy or light chain contamination issues. Denaturing elution is recommended as the first choice due to its higher elution efficiency.

Storage

Store at 4℃ for 12 months.

Precautions

1.Avoid freezing the beads. Store in solution to prevent drying.

2.The average magnetic separation time should be longer than 1 min.

3.Ensure uniform suspension by fully shaking the storage tube before use. Avoid bubbles during operation.

4.Use high-quality tips and test tubes to avoid sample loss due to adhesion.

5.Test the binding of proteins to beads by using the collected supernatant.

6.In IP experiments, the binding affinity of different proteins may vary. Users can select and prepare buffers according to experimental needs.

7.The product is for R&D use only, not for diagnostic procedures, food, drug, household or other uses.

8.Please wear a lab coat and disposable gloves.

Instruction Manual

C0155-Instruction Manual(269 KB)