Mycoplasma Clearance Reagent

E.g., Taking 7.5 mg packing for example:

• High efficiency: Demonstrates strong clearance efficacy against multiple common mycoplasma species.

• Excellent cell compatibility: Effectively removes mycoplasma with minimal impact on cell growth, morphology, and metabolism.

• Broad-spectrum activity: Besides mycoplasma, it also exhibits inhibitory effects against certain common Gram-negative and Gram-positive bacteria

• Easy to use: Simply add to the culture medium to treat contaminated cells; suitable for a wide range of cell types.

Suitable for treating and eliminating mycoplasma contamination during cell culture and is particularly beneficial for valuable or irreplaceable cell lines.

With periodic use, it can effectively prevent re-infection, maintain the stability and purity of the cell culture system, and ensure the accuracy and reproducibility of downstream experiments.

1. Preparation of Stock Solutions

a) Dissolve 5 mg BM-Cyclin-1 in 2 mL sterile ddH2O/PBS to prepare a 2.5 mg/mL (250x) stock solution.

b) Dissolve 2.5 mg BM-Cyclin-2 in 2 mL sterile ddH2O/PBS to prepare a 1.25 mg/mL (250x) stock solution.

Note: The recommended working concentration for BM-Cyclin-1 is 10 µg/mL, and for BM-Cyclin-2 is 5 µg/mL. These concentrations generally do not affect most cell types. If cells are more sensitive, the working concentration may be reduced accordingly.

2. Mycoplasma Elimination Procedure

a) Discard the old culture medium from the contaminated cells and add fresh medium containing 10 µg/mL (1x) BM-Cyclin-1. Incubate at 37 ℃ for 3 days.

b) Discard the medium and add fresh medium containing 5 µg/mL (1x) BM-Cyclin-2. Incubate at 37 ℃ for 4 days.

c) The steps above constitute one complete treatment cycle. It is recommended to repeat two consecutive cycles to ensure complete mycoplasma elimination.

d) After treatment, replace with fresh drug-free medium and culture for 3-5 days to allow cells to recover.

3. Verification of Clearance Efficiency

After treatment, verify the clearance effectiveness using DAPI staining solution (C0163) or another mycoplasma detection kit.

Powder: -20 ℃, 3 years.

Prepared solution: –20 ℃, 6 months.

1.BM-Cyclin-1 and BM-Cyclin-2 stock solutions should be aliquoted and stored at -20 ℃. Avoid repeated freeze-thaw cycles to prevent loss of potency.

2.Avoid combining with other antibiotics during treatment to prevent cellular damage.

3.For particularly sensitive cell types, it is recommended to use half of the originally recommended working concentration and treat for three consecutive cycles.

4.If mycoplasma is not fully eliminated after two cycles, increase the working concentration by approximately 50% for subsequent treatment and continue for one additional cycle.

5.Always use BM-Cyclin-1 and BM-Cyclin-2 in sequence. Do not mix them together.

6.The product is for R&D use only, not for diagnostic procedures, food, drug, household or other uses.

7.Please wear a lab coat and disposable gloves.

• C0192-Instruction Manual(194 KB)