mixed

5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-CR110, SE [5-(and 6)-Carboxyrhodamine 110, succinimidyl ester] *Mixed isomers* is a Fluorescein for peptide and oligonucleotide labeling. Compared to fluorescein labeling reagents such as FITC and FAM, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. They are photostable alternative reagents superior to FITC and FAM.

5(6)-TAMRA (Mixed isomers) (5-(and-6)-Carboxytetramethylrhodamine) is a rhodamine-type fluorescent dye with excitation/emission wavelengths of 545/580 nm. It is commonly employed for labelling oligonucleotides in DNA analysis.

ROX dyes have a strong red fluorescence.Their excitation and emission wavelengths are longer than those of traditional rhodamine dyes.They are used as labels for peptides, proteins, and other biological ligands.5-ROX, 6-ROx, and their mixture of 5(6) -RoX

5(6)-TRITC [Tetramethylrhodamine-5-(and-6) -Isothiocyanate] *Mixed isomers* is used to label antibodies with strong fluorescent dyes

Luminol (Diogenes reagent)(Diogenes reagent) is a versatile chemical, exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid.

5(6)-Aminofluorescein (5(6)-AFM) is a high-purity, high-quality chemical utilized as a molecular probe and a significant fluorescent agent.

5(6)-FAM SE (5(6)-Carboxyfluorescein N-hydroxysuccinimide ester) is an amine-reactive green fluorescent dye. 5(6)-FAM SE is widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine.

5(6)-TAMRA SE (5(6)-Carboxytetramethylrhodamine N-succinimidyl ester) is the amine-reactive, mixed isomer form of TAMRA. 5(6)-TAMRA SE is a dye for oligonucleotide labeling and automated DNA sequencing applications.

Bromo-PADAP is a dye agent utilized in research for the spectrophotometric determination of uranium(VI). This compound exhibits high sensitivity to uranium, with a molar absorptivity of its uranyl complex at 74,000 at 578 nm and pH 7.6. In the presence of mixed masking solutions, only a few ions cause significant interference. By initially extracting uranium into trioctylphosphine oxide and subsequently developing the Bromo-PADAP color in the organic phase, the method becomes specifically targeted to uranium. Detailed procedures for the determination of uranium in water, ores, phosphoric acid and phosphate rocks, thorium oxide, and zirconium oxide are provided in the text.

Difluorocarboxyfluorescein NHS Ester, 6-isomer, is a fluorescent analogue of fluorescein that reacts with amines. It addresses several limitations of fluorescein, offering enhanced photostability and a lower pKa (~4.7 compared to fluorescein's 6.4), resulting in fluorescence that remains largely pH-insensitive within physiological ranges. Although mixed isomers of difluorocarboxyfluorescein NHS ester are often used as routine fluorescent dyes for labeling proteins, peptides, and nucleotides, purifying peptides and nucleotides labeled with the 5(6) isomer can be challenging due to signal broadening during HPLC purification. Peptides and nucleotides labeled with a single isomer typically show improved resolution in HPLC purification, which is often necessary during conjugation processes.

Rhodamine B isothiocyanate (mixed isomers) is an orange-red fluorescent dye exhibiting cell permeability, capable of amine coupling with proteins and antibodies. With excitation/emission wavelengths of 570/595 nm, it is suitable for fluorescence microscopy and flow cytometry.

3-HTC serves as a reversible chemical fluorescent probe that interacts with thiols and disulfides. This compound enables the measurement of GSH GSSH ratios dynamically in vitro and facilitates monitoring of the reversible redox status in whole cell lysates (λ max: 448 nm in its reduced thiolate form, and a λ max 370-410 nm for the oxidized mixed disulfide) [1].

5(6)-ROX, SE is an amine-reactive fluorescent probe that is widely used for oligonucleotide labeling and automated DNA sequencing applications.