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Luminol

Name: Luminol
Brand: TargetMol
SKU: T15792
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T15792Cas No. 521-31-3

Alias Diogenes reagent

Luminol (Diogenes reagent)(Diogenes reagent) is a versatile chemical, exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.

Luminol

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Purity: 99.82%

Catalog No. T15792Alias Diogenes reagentCas No. 521-31-3

Luminol (Diogenes reagent)(Diogenes reagent) is a versatile chemical, exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$37	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.82%

Appearance:Solid

Color:White to Green

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COA HPLC HNMR

Product Introduction

Luminol AI Summary

Luminol demonstrates a diverse range of bioactivities. It acts as an inhibitor of eubacterial tRNA guanine transglycosylase (TGT) from Zymomonas mobilis with a Ki of 36000.0 nM. It also inhibits Aldehyde Dehydrogenase 1 (ALDH1A1) with a potency of 35481.3 nM and RanGTP-induced Rango-Importin beta complex dissociation with a potency of 5804.8 nM. Additionally, Luminol inhibits Marburg Virus entry into cells with a potency of 562.3 nM and is a substrate of Mammalian Selenoprotein Thioredoxin Reductase 1 (TrxR1) with a potency of 44668.4 nM. Moreover, it stimulates Tumor Necrosis Factor-alpha (TNF-alpha) secretion at 59557.2 nM and acts as an agonist of the antioxidant response element (ARE) signaling pathway with a potency of 74978.0 nM. It also inhibits human tyrosyl-DNA phosphodiesterase 1 (TDP1) in the presence of CPT at 1458.1 nM and in the absence of CPT at 29092.9 nM. Furthermore, it inhibits cullin neddylation in a TR-FRET assay with an IC50 of 38400.0 nM. In antiviral assays, Luminol inhibits SARS-CoV-2 induced cytotoxicity of Caco-2 cells at 10 µM after 48 hours, with 9.97% inhibition, and shows inhibition of SARS-CoV-2 3CL-Pro protease at 20 µM concentrations, with inhibition percentages of 156.93% and 29.21%. In VERO-6 cells, it inhibits SARS-CoV-2 induced cytotoxicity at 10 µM after 48 hours with inhibition percentages ranging from -0.06% to -0.16%. Lastly, in an enzymatic assay of human HDAC6, it exhibits 35.23% inhibition with a commercial peptide substrate, but -1.43% inhibition with a custom peptide substrate..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Luminol (Diogenes reagent)(Diogenes reagent) is a versatile chemical, exhibits chemiluminescence, with a striking blue glow, when mixed with an appropriate oxidizing agent.
In vitro	Instructions for use I. Solution preparation 1. Stock solution: Dissolve Luminol in an alkaline solution (such as 0.1 M NaOH or 50 mM Tris-HCl, pH 8.5–9.0), usually at a concentration of 10–50 mM. Note: The powder should be stored in a dry environment at -20°C away from light; the stock solution can be stored at 4°C for a short period of time, and it is recommended to store it at -20°C after aliquoting away from light for a long period of time to avoid repeated freezing and thawing. 2. Working solution: Dilute the stock solution to a final concentration of 0.1–1 mM. (Please adjust the working solution concentration according to experimental requirements) II. Operation steps 1. Chemiluminescent reaction Luminol generates light through peroxidase (such as H₂O₂) or catalyst (such as iron ions) in the presence of peroxide (such as H₂O₂). 1) Reaction system: 2) Add peroxide (such as 0.1–10 mM H₂O₂) and enzyme (such as 1–10 µg/mL HRP) as excitation agents. 3) Detection method: Measure the light signal by a chemiluminescence detector (such as a microplate luminescence reader). Notes: 1. Luminol is sensitive to light and oxygen. Avoid light and air exposure during operation. 2. Use the working solution as soon as possible after preparation to reduce degradation and signal attenuation. 3. Luminol concentration is an important factor affecting the intensity of chemiluminescence (CL). The intensity of luminol chemiluminescence (LCL) depends not only on the concentration of luminol, but also on other factors such as the concentration of oxidants, enzymes, and pH. The maximum recorded CL intensity is at a luminol concentration of 0.3 mM. In the range of 0.01 to 0.3 mM, the CL intensity increases linearly with the increase of luminol concentration. However, further increasing the luminol concentration resulted in a decrease in the luminescence intensity. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Diogenes reagent

Chemical Properties

Molecular Weight	177.16
Formula	C₈H₇N₃O₂
Cas No.	521-31-3
Smiles	NC1=C2C(=O)NNC(=O)C2=CC=C1
Relative Density.	1.433 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 41.67 mg/mL (235.21 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2 mg/mL (11.29 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.6446 mL	28.2231 mL	56.4462 mL	282.2308 mL
5 mM	1.1289 mL	5.6446 mL	11.2892 mL	56.4462 mL
10 mM	0.5645 mL	2.8223 mL	5.6446 mL	28.2231 mL
20 mM	0.2822 mL	1.4112 mL	2.8223 mL	14.1115 mL
50 mM	0.1129 mL	0.5645 mL	1.1289 mL	5.6446 mL
100 mM	0.0564 mL	0.2822 mL	0.5645 mL	2.8223 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc