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Anti-MLKL Polyclonal Antibody
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Catalog No. TMAB-01149
Alias Mlkl, Mixed lineage kinase domain-like protein
Anti-MLKL Polyclonal Antibody is a Rabbit antibody targeting MLKL. Anti-MLKL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MLKL Polyclonal Antibody
Anti-MLKL Polyclonal Antibody
😃Good
Catalog No. TMAB-01149Alias Mlkl, Mixed lineage kinase domain-like protein
Anti-MLKL Polyclonal Antibody is a Rabbit antibody targeting MLKL. Anti-MLKL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-MLKL Polyclonal Antibody is a Rabbit antibody targeting MLKL. Anti-MLKL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Mlkl, Mixed lineage kinase domain-like protein |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | Sample: Lane 1: Human HeLa cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-MLKL (TMAB-01149) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]. |
| Immunogen | Recombinant Protein: protein of mouse MLKL |
| Antigen Species | Mouse |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Necroptosis,SARS Coronavirus,Other Kinases |
| Molecular Weight | Theoretical: 54 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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