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NKH477

Name: NKH477
Brand: TargetMol
SKU: T16332
Price: 149 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16332Cas No. 138605-00-2

Alias NKH 477, Colforsin dapropate hydrochloride

NKH477 (Colforsin dapropate hydrochloride) is a derivative of forskolin with antidepressant activity. It inhibits ACh-induced Ca2+ mobilization by acting on ionomycin-sensitive storage sites. NKH477 is an adenylyl cyclase activator with bronchodilatory effects, inhibiting the production of CTL, T-cell proliferation in mixed lymphocyte reactions (MLR), as well as IL-2 production and mitogen responses.

NKH477

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Purity: 98%

Catalog No. T16332Alias NKH 477, Colforsin dapropate hydrochlorideCas No. 138605-00-2

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$149	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98%

Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	NKH477 (Colforsin dapropate hydrochloride) is a derivative of forskolin with antidepressant activity. It inhibits ACh-induced Ca2+ mobilization by acting on ionomycin-sensitive storage sites. NKH477 is an adenylyl cyclase activator with bronchodilatory effects, inhibiting the production of CTL, T-cell proliferation in mixed lymphocyte reactions (MLR), as well as IL-2 production and mitogen responses.
In vitro	NKH477 (0.1-1.0 μM) inhibits ACh induced contraction in a concentration-dependent manner by attenuating ACh induced Ca2 + mobilization and reducing the sensitivity of the contractile mechanism to Ca2 +. [1]
In vivo	In experimental dogs, intravenous administration of NKH477 (1-30 μg/kg) resulted in an increase in left ventricular dP/dtmax, coronary and femoral blood flow, heart rate, and myocardial oxygen consumption, as well as a decrease in blood pressure in anesthetized dogs. NKH477 is a useful insulin dilator for the treatment of heart failure. [2]
Synonyms	NKH 477, Colforsin dapropate hydrochloride

Chemical Properties

Molecular Weight	546.09
Formula	C₂₇H₄₄ClNO₈
Cas No.	138605-00-2
Smiles	O[C@@]12[C@]3(C)[C@@]([C@H](OC(CCN(C)C)=O)[C@H](OC(C)=O)[C@@]1(C)O[C@](C=C)(C)CC2=O)(C(C)(C)CC[C@@H]3O)[H].Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (146.5 mM), Sonication is recommended.

H2O: 10 mg/mL (18.31 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (9.16 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8312 mL	9.1560 mL	18.3120 mL	91.5600 mL
5 mM	0.3662 mL	1.8312 mL	3.6624 mL	18.3120 mL
10 mM	0.1831 mL	0.9156 mL	1.8312 mL	9.1560 mL

DMSO

	1mg	5mg	10mg	50mg
20 mM	0.0916 mL	0.4578 mL	0.9156 mL	4.5780 mL
50 mM	0.0366 mL	0.1831 mL	0.3662 mL	1.8312 mL
100 mM	0.0183 mL	0.0916 mL	0.1831 mL	0.9156 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc