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Anti-Phospho-MLKL (Ser345) Antibody (2S387)
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Catalog No. TMAB-01454
Anti-Phospho-MLKL (Ser345) Antibody (2S387) is a Rabbit antibody targeting Phospho-MLKL (Ser345). Anti-Phospho-MLKL (Ser345) Antibody (2S387) can be used in WB,IP.
Anti-Phospho-MLKL (Ser345) Antibody (2S387)
Anti-Phospho-MLKL (Ser345) Antibody (2S387)
😃Good
Catalog No. TMAB-01454
Anti-Phospho-MLKL (Ser345) Antibody (2S387) is a Rabbit antibody targeting Phospho-MLKL (Ser345). Anti-Phospho-MLKL (Ser345) Antibody (2S387) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $153 | 7-10 days | |
| 50 μL | $272 | 7-10 days | |
| 100 μL | $487 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IP Reactivity: Mouse (predicted:Human) |
| Ig Type | IgG |
| Clone | 2S387 |
| Reactivity | Mouse (predicted:Human) |
| Verified Activity | 1. Western blot analysis of Phospho-MLKL (S345) on L929 cell lysates. Lane 1: L929 cells, whole cell lysate, 10 μg/lane. Lane 2: L929 cells were treated with 20 uM Z-VAD for 30 minutes, then added 20 ng/ml mTNF-alpha and 100 nM SM-164 for 4 hours, whole cell lysates, 10 μg/lane. 2. 4% Paraformaldehyde-fixed L-929 (treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic, and 20 µM z-VAD for 8 h) (M) cell; Triton X-100 at RT for 20 min; Antibody incubation with (phospho-MLKL (Ser345)) monoclonal Antibody, unconjugated (TMAB-01454) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-2000; IP: 1:10-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]. |
| Immunogen | A synthesized peptide: mouse Mlkl around the phosphorylation site of S345 |
| Antigen Species | Mouse |
| Gene ID | 74568 |
| Uniprot ID | |
| Biology Area | Necroptosis,SARS Coronavirus,Other Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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