TO-PRO 1

TO-PRO 1, a DNA-binding fluorochrome, is attached to the surface of Feraheme (FH) nanoparticles (NP) to create a fluorochrome-functionalized NP. It binds DNA via intercalation and serves as a crucial fluorochrome for identifying necrotic cells [1]. For storage, it is essential to protect it from light.

Z-Gly-Pro-Arg-4MβNA is a thrombin substrate that, upon cleavage by thrombin, releases 4-methoxy-2-naphthylamine (4MβNA). This liberated 4MβNA reacts with 5-nitrosalicylaldehyde to form an insoluble yellow fluorescent product, indicating the site of thrombin activity [1].

Z-Gly-Pro-Arg-AMC hydrochloride is a fluorescent substrate for both trypsin and cathepsin K, utilized to assay their respective enzymatic activities [1] [2].