MitoSOX Red is a novel fluorescent probe that is specific and cell membrane-permeable and targets mitochondria in living cells.MitoSOX Red is oxidized by superoxide upon entry into the mitochondria and is not oxidized by other ROS or RNS-generating systems.MitoSOX Red then binds to nucleic acids in the mitochondrion and produces a strong red fluorescence.MitoSOX Red can be used as a fluorescent indicator for the specific detection of superoxide.MitoSOX Red can be used as a fluorescent indicator for the specific detection of superoxide. MitoSOX Red can be used as a fluorescent indicator to specifically detect superoxide. Superoxide dismutase (SOD) prevents the oxidation of MitoSOX Red.
ER-Tracker Green (ERTG) is a cell permeable live cell stain for live cell imaging.ER-Tracker Green has an excitation wavelength of 504 nm and an emission wavelength of 511 nm.
Nile Red (Nile Blue A oxazone) is a strongly fluorescent stain used for the detection of intracellular lipid droplets in the presence of a hydrophobic environment.
DiI (DiIC18(3)) is a lipophilic membrane dye commonly used as a long-term tracer for neurons and other cells. DiI fluoresces very weakly before it enters the cell membrane, and only after it enters the cell membrane can it be excited to emit a strong orange-red fluorescence (λex=549 nm, λem=565 nm).
LysoTracker Red (LysoTracker Red DND-99) is a fluorescent probe compound for lysosomal capture, a transport substrate for P-glycoprotein, with a maximum excitation emission wavelength of 577 590 nm.LysoTracker Red can be used for the long-term tracking of acidic vesicles.
BODIPY 505 515 is a lipophilic fluorescent probe that localises to intracellular liposomes and has been used to label lipid droplets.BODIPY 505 515 has been used in electron microscopy, epifluorescence microscopy, confocal microscopy, and flow cytometry applications for various algal species.
MKT-077 (FJ-776) is a cationic rhodacyanine dye that demonstrates antiproliferative activity against cancer cell lines (EC50s: 1.4-2.2 μM in vitro) through its ability to inhibit members of the Hsp70 family of molecular chaperones.
Rhodamine 6G (Basic Red 1) is a rhodamine analog useful in Pgp efflux assays. It can be widely used in characterizing the kinetics of MRP1- mediated efflux as a laser dye and potential mitochondrial probe.
Acridine Orange 10-Nonyl Bromide is a mitochondrial fluorescent probe that binds to cardiolipin in the inner mitochondrial membrane, dependent on the mitochondrial membrane potential, with an excitation wavelength of 490 nm and an emission wavelength of 510–525 nm.
2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.
MitoPerOx is a ratiometric fluorescent probe that can be used to assess changes in lipid peroxidation within mitochondria. It is composed of a BODIPY fluorophore conjugated via a dienyl link to a triphenylphosphonium cation component that drives its accumulation in mitochondria. MitoPerOx displays an excitation maximum of 495 nm and exhibits a shift in emission maxima from 590 to 520 nm upon mitochondrial lipid peroxidation, enabling determination of ratiometric measurements of lipid peroxidation in live cells.
Endoplasmic reticulum dye 1 is a highly effective live cell imaging agent used to detect exocytotic events at the plasma membrane. The compound exhibits minimal cytotoxicity and is resistant to photobleaching, making it ideal for visualizing short- and long-term processes.
FIM-1, a fluorescent probe for protein kinase C (PKC), facilitates mitochondrial staining and serves as an ATP-competitive inhibitor at the catalytic site of PKC, effectively inhibiting its activity [1].
ICG-OSu, also known as ICG NHS ester, is a near-infrared fluorescent agent commonly utilized for the development of in vivo imaging probes due to its amine-reactive properties.
HKSOX-1m (5 6-mixture) is a mitochondria-targeting O2 fluorescent probe with excitation emission wavelengths of 509 534 nm (green), offering exceptional selectivity and sensitivity for O2 across a wide pH range and in the presence of strong oxidants and prevalent reductants within cells.
HKSOX-1m hydrobromide (5 6-mixture), a mitochondria-targeted O2•− fluorescent probe (Ex Em=509 534nm; green), demonstrates exceptional selectivity and sensitivity for O2•− across a wide pH spectrum, robustly distinguishing it from strong oxidants and prevalent cellular reductants [1].