NBD-PE is a lipid dye used primarily as a probe to study lipid organisation and dynamics within cell membranes and also to study membrane fusion by fluorescence resonance energy transfer (FRET), Ex=463 nm, Em=536 nm.

DQ-BSA-RED is a red-fluorescently labeled bovine serum albumin derivative specifically designed to monitor lysosomal proteolytic activity. DQ-BSA-RED exhibits excitation and emission maxima at 590 nm and 620 nm, respectively, and contains multiple dye moieties resulting in fluorescence self-quenching until proteolytic cleavage occurs within lysosomes, upon which bright fluorescence is emitted. DQ-BSA-RED therefore enables the visualization and quantification of lysosomal functionality in live-cell assays, while inactive lysosomes fail to generate fluorescence, providing a valuable tool for studying autophagy and intracellular degradation processes.

Lyso BD-1 predominantly colocalizes with lysosomes and lipid droplets, exhibiting exceptional photocytotoxicity (5.57 μM) against triple-negative breast cancer cells under white light irradiation. The emission wavelength of Lyso BD-1 is 560 nm.

HCy-Lyso is a lysosome-specific fluorescent probe specifically designed for the detection of hydroxyl radicals.

HMSiR680-Me is a bright and stable fluorescent dye designed for multicolor, time-lapse, and super-resolution imaging to observe acidic organelles. It is suitable for overnight time-lapse experiments and rapid continuous super-resolution microscopy imaging, maintaining imaging quality for at least 1000 frames. HMSiR680-Me can also be used with silicon rhodamine dyes in multiplexed super-resolution microscopy experiments, enabling the visualization of interactions between the mitochondrial inner membrane and lysosomes using a single excitation laser and simultaneous depletion technique.

Lyso Flipper-TR 29, a Flipper probe, specifically labels lysosomes and late endosomes. It achieves entry into these organelles by transient deprotonation, allowing it to cross their membranes in a neutral form [1].

CTB Probe-1 (probe 1) serves as a small-molecule, lysosome-targeting fluorogenic probe designed for fluorescence imaging within living cells [1].

Green DND-26 is a lysosomal probe tagged with green fluorescence, displaying peak excitation and emission wavelengths at 504/511 nm. It consists of a fluorescein group and attached weak bases that can easily permeate the cell membrane and predominantly accumulate in spherical organelles. This compound is effective for examining lysosomal biosynthesis and associated pathogenesis [1].