Lipid droplets

BODIPY 493/503 (Pyrromethene 546) is a lipophilic fluorescent probe with Ex/Em of 493/503 nm. BODIPY 493/503 localizes to polar lipids and can be used to label cellular neutral lipid contents and for live and fixed cell applications.

The dry dye Oil red O is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections.

C-12 NBD cholesterol, a cholesterol derivative with a hydrophilic fluorophore tag (NBD: Ex=465 nm, Em=535 nm), is utilized to investigate cholesterol utilization and metabolism [1].

Bodipy TR alkyne, a boron dipyrromethene fluorophore for the ROX (Texas Red) channel, is versatile for applications in microscopy, fluorescence polarization measurements, and other uses. This derivative serves as a terminal alkyne in copper-catalyzed click chemistry.

BODIPY-C12, a boron-dipyrromethene derivative with a lipophilic tail (excitation 490 nm), is a hydrophobic molecule sensitive to the surrounding environment and can be used to quantify viscosity [1].

BETA-1, a twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule, exhibits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria, facilitating the dual-color imaging of these organelles both in vivo and in vitro [1].

LipidGreen 2, a second-generation small molecule probe for lipid imaging, offers an enhanced fluorescence signal compared to the original LipidGreen. It selectively stains neutral lipids in cells and fat deposits in live zebrafish [1].

FG 488 DHPE, a lipid-coupled fluorochrome, is used as the fluorophore Oregon Green 488. It monitors the acidification of lipid vesicles with λex λem=508 534 nm and is also utilized for quantifying Hv1-induced proton translocation with the same excitation emission wavelengths [1] [2].

BODIPY FL DHPE, a green-fluorescent phospholipid probe, specifically targets and labels the lipid 1,2-dihexadecanoyl-sn-glycero-phosphoethanolamine (DHPE). When labeled, these liposomes can subsequently be internalized through membrane fusion. This compound is particularly useful for studying both membrane surfaces and the process of membrane fusion. (λ ex =505 nm, λ em =511 nm) [1].

Sudan Black B, a fat-soluble diazo dye, serves as a histochemical stain, particularly effective for staining neutral triglycerides and lipids [1].

PTZ-LD, a phenothiazine-based fluorescent probe, specifically detects lipid droplets (LDs) with apparent emissivity (Ex Em=488 570-620 nm) and is utilized in diabetic cataract (DC) research [1].

BODIPY TR Cadaverine, a derivative of cadaverine, is a red fluorescent dye used in a highly sensitive and robust fluorescent displacement assay. It binds strongly to native LPS, specifically recognizing lipid A, and is competitively displaced by compounds that have an affinity for lipid A [1] [2].

BODIPY 500 510 C1, C12, a derivative of the robust BODIPY dye family, exhibits strong ultraviolet absorption and emits fluorescence with a high quantum yield at a sharp peak. This dye's effectiveness is minimally affected by environmental factors such as polarity or pH, and it maintains stability under various physiological conditions. BODIPY 500 510 C1, C12 has the unique ability to penetrate cell membranes and specifically target polar lipids within cells, effectively staining lipid droplets. This property makes it suitable for labeling both live and fixed cells [1]. It has a maximum excitation emission wavelength of 500 510 nm [1]. For optimal preservation, it should be protected from light and stored at -20°C.

C6-NBD Sphinganine, a fluorescent sphinganine analog, serves as a dye for labeling fatty acids [1].