ER Flipper-TR 28, a small molecule fluorophore flipper probe, enables imaging of membrane tension within the endoplasmic reticulum (ER). This compound is characterized by a pentafluorophenyl group and reacts with protein thiolate on the cytoplasm-facing surface of the ER [1].

ER-Tracker dye, a derivative of BODIPY series dyes coupled with Glibenclamide, exhibits high selectivity for the endoplasmic reticulum and is non-toxic to cells at low concentrations. This environmentally sensitive probe retains part of its fluorescence after formaldehyde treatment and features high fluorescence life and a good extinction coefficient. Glibenclamide, an ATP-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker, binds in the endoplasmic reticulum. However, ER-Tracker is not suitable for staining cells after fixation [1].

ER-Tracker dye is a derivative of BODIPY conjugated with Glibenclamide, displaying high selectivity for the endoplasmic reticulum. At low concentrations, it remains non-toxic to cells and serves as an environmentally sensitive probe that retains some fluorescence after formaldehyde treatment. It is characterized by its high fluorescence lifetime and strong extinction coefficient. Glibenclamide acts as an ATP-dependent K+ channel blocker (Kir6, KATP) and a CFTR Cl- channel blocker, binding within the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Ex/Em = 587/615 nm.

The PNT sensor PS3 is an innovative fluorescent peroxynitrite (PNT) sensor characterized by high sensitivity and selectivity. It primarily accumulates in the endoplasmic reticulum of the cell membrane, where it produces fluorescent products through oxidative cleavage by PNT.

FerroOrange is a fluorescent probe designed to detect labile ferrous ions, localizing specifically to the endoplasmic reticulum. Upon interaction with ferrous ions, it irreversibly emits an orange fluorescence. The excitation/emission wavelengths are 542/572 nm.