ribonuclease a

Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation.

Ribonuclease T1 (Rnase T1), widely utilized in biochemical research, is an endonuclease targeting single-stranded RNA. It cleaves the phosphodiester bond between 3'-guanosine residues and adjacent nucleoside 5'-OH groups, thereby producing 3'-GMP terminal oligonucleotides, and forms nucleoside 2', 3'-cyclic phosphoric acid intermediates in the process [1].

Ribonuclease H is an enzyme that degrades the RNA moiety of DNA-RNA hybrids [1].

RNase A, Bovine Pancreas (Ribonuclease A) (DNase & Protease Free) is a ribonuclease and a widely used endonuclease that functions by specifically hydrolyzing cytosine or uracil residues in RNA. It is commonly employed in cell cycle analysis experiments.

ß-Thujaplicinol, a hydroxylated thujaplicinone isolated from western red cedar heartwood, has antiviral activity, inhibits hepatitis B virus replication by blocking viral ribonuclease H activity, and can be used in the study of viral infections.

F1-RIBOTAC is a ribonuclease-targeting chimera (RIBOTAC) that reduces QSOX1-amRNA expression through an RNase L-dependent mechanism. It is applicable in cancer research. (Pink: RNA ligand; Black: linker; Blue: RNase L ligand.)

2-5A (5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine) pentasodium is a 5'-triphosphorylated (2',5') oligo-adenylate. Acting as an immune mediator, 2-5A pentasodium enhances the activity of RNase L. It degrades viral mRNA and inhibits protein synthesis by binding to RNase L and activating its ribonuclease function. This compound is useful in research related to respiratory syncytial virus (RSV) and cancer.

DSA8 is a type II kinase inhibitor with IRE1 ribonuclease regulatory activity. It is applicable for research in cancer and metabolic diseases, such as diabetes.

Fomivirsen sodium (ISIS-2922 sodium) is a 21-base phosphorothioate oligodeoxynucleotide that is complementary to the mRNA of the major immediate early region (IE2) of human cytomegalovirus (CMV). It inhibits viral replication by cleaving the target mRNA through the ribonuclease H (RNase H) mechanism. Following intravitreal administration, it can be used in research related to cytomegalovirus retinitis associated with AIDS.

4μ8C (IRE1 Inhibitor III) is a highly selective and potent inhibitor of IRE1α ribonuclease (RNase) activity (IC₅₀ ≈ 6–10 μM). By forming a covalent bond with the RNase active site of IRE1α, 4μ8C specifically inhibits its ribonuclease activity, thereby blocking the splicing of XBP1 mRNA. 4μ8C can be used in research on endoplasmic reticulum stress, the unfolded protein response, immune regulation, and tumor and metabolic diseases.

CI-39, a natural antiviral product, serves as a non-nucleoside reverse transcriptase inhibitor (NNRTI) targeting wild type HIV-1. It exhibits an effective concentration (EC50) of 3.40 µM against the virus, while its cytotoxic concentration (CC50) exceeds 30 µM. This compound notably impedes HIV-1 reverse transcriptase (RT) DNA polymerase and ribonuclease H activities[1].

MYC-RIBOTAC, a ribonuclease-targeting chimera (RIBOTAC) specific to the MYC internal ribosome entry site (IRES), comprises an MYC mRNA-binding element tethered to a small molecule that recruits and activates RNase L1. This interaction promotes the degradation of MYC mRNA, subsequently reducing MYC protein levels and inducing apoptosis, thereby presenting potential applications in anticancer research [1].

Aurintricarboxylic acid (NSC-4056) is a strong inhibitor of topoisomerases and other nucleases. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme.

S Tag Peptide acetate is a short oligopeptide derived from pancreatic ribonuclease A, encoded by pCITE-3 and -4 series vectors for in vitro translation and by pET-29 and -30 vectors for expression in E. coli. S Tag Peptide acetate is fused at the N-terminus of target proteins and contains a multiple cloning site region available in all three reading frames, enabling efficient protein expression, purification, and functional studies.

Ribonuclease T2, Aspergillus oryzae, is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on improving the kinetics of enzyme reactions, substrate selectivity, and activity under extreme conditions (e.g., low or high pH). By introducing stimuli-responsive modifications to these enzymes, dynamic control of activity is also made possible.

RNase B, Bovine Pancreas, is the N-glycosylated form of bovine pancreatic ribonuclease, structurally analogous to RNase A. It assists in polypeptide chain folding and functions similarly to a molecular chaperone.

RNase A (10mg/mL, DNase free) is a ribonuclease used to catalyze RNA degradation and lacks DNase activity. It is commonly utilized in cell cycle analysis experiments.