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Anti-Ribonuclease 3/ECP Polyclonal Antibody
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Catalog No. TMAB-12250
Anti-Ribonuclease 3/ECP Polyclonal Antibody is a Rabbit antibody targeting Ribonuclease 3/ECP. Anti-Ribonuclease 3/ECP Polyclonal Antibody can be used in WB,ICC/IF.
Anti-Ribonuclease 3/ECP Polyclonal Antibody
Anti-Ribonuclease 3/ECP Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-12250
Anti-Ribonuclease 3/ECP Polyclonal Antibody is a Rabbit antibody targeting Ribonuclease 3/ECP. Anti-Ribonuclease 3/ECP Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Ribonuclease 3/ECP Polyclonal Antibody is a Rabbit antibody targeting Ribonuclease 3/ECP. Anti-Ribonuclease 3/ECP Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#TMAB-12250) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). 2. RNASE3 Antibody (C-term) (Cat. #TMAB-12250) western blot analysis in NCI-H460 cell line lysates (35 μg/lane). This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow). 3. Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term) (Cat. #TMAB-12250). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&l (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35 ug. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic granule. Matrix of eosinophil's large specific granule. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | ECP is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis, and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases |
| Immunogen | KLH conjugated synthetic peptide: human Ribonuclease 3 |
| Antigen Species | Human |
| Gene Name | RNASE3 |
| Gene ID | |
| Protein Name | Eosinophil cationic protein |
| Uniprot ID | |
| Function | Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity. |
| Molecular Weight | Theoretical: 16 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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