Anti-Ribonuclease Inhibitor Polyclonal Antibody 2 is a Rabbit antibody targeting Ribonuclease Inhibitor. Anti-Ribonuclease Inhibitor Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (TMAB-12251) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Blank control (blue): Hela Cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Ribonuclease Inhibitor antibody, Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (TMAB-12251) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Ribonuclease Inhibitor

Human

RNH1

Ribonuclease inhibitor