store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 41.00 |
Description | Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation. |
In vitro |
The most common application of Ribonuclease A is to remove RNA during plasmid DNA or genomic DNA preparation. Preparation of Ribonuclease A Stock Solution:Prepare a 10 mg/mL Ribonuclease A stock solution using 10 mM sodium acetate (pH 5.2).Heat at 100°C for 15 minutes. Cool to room temperature, add 1/10 (v/v) 1 M Tris-HCl (pH 7.4), and adjust the pH to 7.4.Store at -20°C for stable storage for up to 2 years. Note: Boiling the Ribonuclease A solution under neutral conditions may lead to the precipitation of RNase. Boiling at a lower pH, if precipitation occurs, may be due to the presence of protein impurities. In such cases, the precipitate can be removed by high-speed centrifugation (13,000 rpm). |
Synonyms | EC 3.1.27.5, RNase A |
Molecular Weight | N/A |
CAS No. | 9001-99-4 |
store at low temperature
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 80 mg/mL, Sonication is recommended.
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Ribonuclease A 9001-99-4 EC 3.1.27.5 RNase A inhibitor inhibit