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Ribonuclease A
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Catalog No. T78345Cas No. 9001-99-4
Alias RNase A, EC 3.1.27.5
Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation.
Ribonuclease A
Copy Product Info🥰Excellent
Catalog No. T78345Alias RNase A, EC 3.1.27.5Cas No. 9001-99-4
Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 mg | $41 | In Stock | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Potency:60 U/mg
Appearance:Solid
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation. |
| In vitro | The most common application of Ribonuclease A is to remove RNA during plasmid DNA or genomic DNA preparation. Preparation of Ribonuclease A Stock Solution:Prepare a 10 mg/mL Ribonuclease A stock solution using 10 mM sodium acetate (pH 5.2).Heat at 100°C for 15 minutes. Cool to room temperature, add 1/10 (v/v) 1 M Tris-HCl (pH 7.4), and adjust the pH to 7.4.Store at -20°C for stable storage for up to 2 years. Note: Boiling the Ribonuclease A solution under neutral conditions may lead to the precipitation of RNase. Boiling at a lower pH, if precipitation occurs, may be due to the presence of protein impurities. In such cases, the precipitate can be removed by high-speed centrifugation (13,000 rpm). |
| Synonyms | RNase A, EC 3.1.27.5 |
| Cas No. | 9001-99-4 |
| Sequence | bAla-DL-His |
| Sequence Short | XH |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | H2O: 80 mg/mL, Sonication is recommended. |
Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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