BETA-1 is the first integrated molecule combining intramolecular charge transfer and aggregation-induced emission. It can generate cyan and red fluorescent signals in lipid droplets and mitochondria, respectively, and is suitable for dual-color simultaneous imaging of lipid droplets and mitochondria in both in vivo and in vitro systems.

BETA-1 can be employed for the imaging of lipid droplets and mitochondria in living cells[1]. Its fluorescence emission parameters are as follows: for the cyan fluorescence channel, the excitation wavelength is 405 nm and the emission wavelength ranges from 430 to 500 nm; for the red fluorescence channel, the excitation wavelength is 561 nm and the emission wavelength ranges from 600 to 700 nm.
HeLa cells were routinely cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified atmosphere of 5% CO₂ and 95% air. HeLa cells were seeded into glass-bottom 96-well plates at a density of 5.0×10³ cells per well and incubated for 24 h. For cell imaging experiments, live HeLa cells were stained with 5 μM BETA-1 solution at 37 °C for 30 min. Fluorescence emission signals of the corresponding channels were collected using 405 nm and 561 nm lasers as excitation sources, respectively.

DMSO: 20 mg/mL (28.34 mM), Sonication is recommended.