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Sudan Black B
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Catalog No. T87451Cas No. 4197-25-5
Sudan Black B is a lipophilic diazo dye commonly used in histochemistry to stain neutral lipids, phospholipids, lipoproteins, and lipofuscin within the cytoplasm, facilitating studies of lipid and cellular metabolism.
Sudan Black B
😃Good
Catalog No. T87451Cas No. 4197-25-5
Sudan Black B is a lipophilic diazo dye commonly used in histochemistry to stain neutral lipids, phospholipids, lipoproteins, and lipofuscin within the cytoplasm, facilitating studies of lipid and cellular metabolism.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 g | $41 | Inquiry | Inquiry | |
| 10 g | $56 | Inquiry | Inquiry |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Chemical Properties
| Description | Sudan Black B is a lipophilic diazo dye commonly used in histochemistry to stain neutral lipids, phospholipids, lipoproteins, and lipofuscin within the cytoplasm, facilitating studies of lipid and cellular metabolism. |
| In vitro | Sudan Black B is also applicable to frozen samples, making it suitable for combined use with SA-β-gal in detecting cellular senescence. Sudan Black B is an intensely lipophilic reagent with high affinity for the lipid components of lipofuscin. Under optical microscopy, Sudan Black B-lipofuscin positive reactions appear as blue-black intracellular granules in cells and frozen tissues, while exhibiting brown-black granules in formalin-fixed paraffin-embedded (FFPE) tissues [1]. |
| Molecular Weight | 456.54 |
| Formula | C29H24N6 |
| Cas No. | 4197-25-5 |
| Smiles | N(=NC1=CC=C(N=NC2=CC=C3NC(NC4=CC=CC2=C43)(C)C)C=5C=CC=CC15)C=6C=CC=CC6 |
| Color | Black |
| Appearance | Solid |
Storage & Solubility Information
| Storage | keep away from direct sunlight | store at -20°C | Shipping with blue ice. | ||||||||||
| Solubility Information | H2O: < 1 mg/mL (insoluble) DMSO: 1 mg/mL (2.19 mM), Sonication is recommended. | ||||||||||
Solution Preparation Table | |||||||||||
DMSO
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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