staining

Ponceau S (Acid Red 112) is an azo protein dye commonly used as a stain in spot blotting assays for protein quantification.

Amido Black 10B (Naphthol Blue Black) is a highly toxic azo dye used for staining proteins on polyacrylamide gels and agarose gels.

Direct Red 80 (Sirius Red), a tetra azo dye, is used to detect amyloid and enhance the birefringence of collagen fibers in tissue sections.

Fuchsine base monohydrochloride (Rosaniline Base monohydrochloride) is a magenta dye and uses for acid-fast staining with carbol-fuchsin.

Methyl blue is a triaminotriphenylmethane dye. It is used as an antiseptic dye in the polychrome staining method and has applications in histological and microbiological staining solutions.

Nitro blue tetrazolium chloride (NBT), an NADPH-diaphorase substrate (IC50 = 3-4 M) and well-known scavenger of superoxide anions, competitively inhibits nitric oxide synthase. Additionally, NBT, as a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.

Solvent Blue 35 (Sudan Blue II) is a dye utilized for coloring hydrocarbon-based and alcoholic solvents, as well as staining triglycerides in animal tissues.

TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.

TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry and as a visualizing reagent in ELISA.

1-Naphthyl acetate is usually used in a rapid staining method for identification of macrophages. 1-Naphthyl acetate is a potent chromogenic substrate for the detection of erythrocyte acetylcholinesterase (AChE) activity. It has the potential to detect organophosphorus pesticide poisoning.

Pyronine B (NSC 44690) is a fluorophore with low relative fluorophore brightness and a small hydrophobic (SH) protein channel inhibitor, which is used for staining of bacteria and mycobacteria and in the spectrophotometric determination of ultra-trace ruthenium.