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Thiazole orange dimer YOYO-1 is a green fluorescent dye used for DNA staining. It is a tetracationic homodimer of oxazole yellow (YO) and is typically provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) exhibits low fluorescence quantum yield, but upon binding to double-stranded DNA through diintercalation, its fluorescence intensity increases by 3200 times (λmax 489 nm; λmax 509 nm).

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 mg | $1,520 | 6-8 weeks | 6-8 weeks | |
| 50 mg | $1,980 | 6-8 weeks | 6-8 weeks | |
| 100 mg | $2,500 | 6-8 weeks | 6-8 weeks |
| Description | Thiazole orange dimer YOYO-1 is a green fluorescent dye used for DNA staining. It is a tetracationic homodimer of oxazole yellow (YO) and is typically provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) exhibits low fluorescence quantum yield, but upon binding to double-stranded DNA through diintercalation, its fluorescence intensity increases by 3200 times (λmax 489 nm; λmax 509 nm). |
| Synonyms | YOYO1, YOYO 1 |
| Molecular Weight | 1270.64 |
| Formula | C49H58I4N6O2 |
| Cas No. | 143413-85-8 |
| Smiles | CN(C1=CC=CC=C1O/2)C2=C/C3=CC=[N+](C4=C3C=CC=C4)CCC[N+](C)(C)CCC[N+](C)(C)CCC[N+]5=CC=C(C6=C5C=CC=C6)/C=C7N(C8=CC=CC=C8O\7)C.[I-].[I-].[I-].[I-] |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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