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Leishman's stain
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Catalog No. T78459Cas No. 12627-53-1
Leishman's stain is a vital dye for staining peripheral blood and bone marrow smears, which appear pale bluish-grey to deep blue when viewed under an oil-immersion lens [1].
Leishman's stain
Leishman's stain
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Catalog No. T78459Cas No. 12627-53-1
Leishman's stain is a vital dye for staining peripheral blood and bone marrow smears, which appear pale bluish-grey to deep blue when viewed under an oil-immersion lens [1].
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Leishman's stain is a vital dye for staining peripheral blood and bone marrow smears, which appear pale bluish-grey to deep blue when viewed under an oil-immersion lens [1]. |
| In vitro | Prepare a stock solution of Sorensen's buffer by dissolving 9.5g/L of Na2HPO4•2H2O (Solution A) and 9.1g/L of KH2PO4 (Solution B). Apply Leishman stain to the smear for 2 minutes, followed by the addition of twice the volume of Sorensen buffer (pH 6.8). Gently mix the contents on the slide for 15 minutes. Finally, rinse the smear with running water and allow it to dry for 5 minutes. (Guidelines: See our recommended protocol. Adapt as necessary for your specific requirements) [1]. |
| Cas No. | 12627-53-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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