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Solvent Blue 35

Name: Solvent Blue 35
Brand: TargetMol
SKU: T19057
Rating: 4.5 (1 reviews)

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Catalog No. T19057Cas No. 17354-14-2

Alias Sudan Blue II, Oil Blue 35

Solvent Blue 35 (Sudan Blue II) is a dye utilized for coloring hydrocarbon-based and alcoholic solvents, as well as staining triglycerides in animal tissues.

Solvent Blue 35

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Purity: 98.40%

Catalog No. T19057Alias Sudan Blue II, Oil Blue 35Cas No. 17354-14-2

Solvent Blue 35 (Sudan Blue II) is a dye utilized for coloring hydrocarbon-based and alcoholic solvents, as well as staining triglycerides in animal tissues.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
5 g	$29	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:98.40%

Appearance:Solid

Color:Purple

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	Solvent Blue 35 (Sudan Blue II) is a dye utilized for coloring hydrocarbon-based and alcoholic solvents, as well as staining triglycerides in animal tissues.
Cell Research	Instructions 1. Solution preparation Solvent Blue 35 is dissolved in isopropanol or acetone. The appropriate dissolution concentration is usually in the range of 0.1-1.0% (w/v). The specific concentration should be adjusted according to the experimental requirements. 2. Tissue staining 1. In tissue staining experiments, Solvent Blue 35 is often used to stain triglycerides in animal tissues. During the staining process, tissue samples are usually dehydrated, transparentized, and other treatments. 2. Add the dissolved dye solution to tissue sections or tissue samples. The staining time is usually 30 minutes to 1 hour. The specific time can be optimized according to the experimental requirements. 3. After staining, the tissue sample can be observed under a microscope. Solvent Blue 35 can bind to lipid components and show significant blue fluorescence. 3. Lipid detection 1. Solvent Blue 35 is often used to detect the accumulation of lipids (such as triglycerides). During the staining process, it can bind to the fatty acid part of the triglyceride molecule to produce color changes or fluorescent signals. 2. By using a microscope or other imaging equipment, researchers can observe the accumulation of lipids in cells or tissues. IV. Cleaning and observation: 1. After staining, use an appropriate cleaning solution to remove unbound dye to avoid affecting the experimental results. Generally, a solution containing a small amount of organic solvent or water is used for washing. 2. Use a microscope to examine the sample. The blue staining effect of Solvent Blue 35 can clearly mark the location of triglycerides. Note:The Solvent Blue 35 dye solution should be stored in a cool and dry place and avoid exposure to strong light. Unused dye solution should avoid repeated freezing and thawing. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Synonyms	Sudan Blue II, Oil Blue 35

Chemical Properties

Molecular Weight	350.45
Formula	C₂₂H₂₆N₂O₂
Cas No.	17354-14-2
Smiles	CCCCNc1ccc(NCCCC)c2C(=O)c3ccccc3C(=O)c12
Relative Density.	1.179 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (114.14 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8535 mL	14.2674 mL	28.5347 mL	142.6737 mL
5 mM	0.5707 mL	2.8535 mL	5.7069 mL	28.5347 mL
10 mM	0.2853 mL	1.4267 mL	2.8535 mL	14.2674 mL
20 mM	0.1427 mL	0.7134 mL	1.4267 mL	7.1337 mL
50 mM	0.0571 mL	0.2853 mL	0.5707 mL	2.8535 mL
100 mM	0.0285 mL	0.1427 mL	0.2853 mL	1.4267 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc