BP Fluor 430 DBCO reacts with azides through a copper-free click chemistry reaction to create a stable triazole, eliminating the need for a Cu catalyst or high temperatures. In applications where copper presence is a concern, BP Fluor 430 DBCO serves as an ideal substitute for copper in fluorescent alkyne requirements. BP Fluor 430 is a bright, photostable green fluorescent probe, optimally excited near its absorption maximum at 432 nm. Its emission peak at 539 nm remains unaffected across a broad pH range. Due to high background interference, BP Fluor DBCO reagents are unsuitable for staining intracellular components of fixed and permeabilized cells.