Light Green SF Yellowish (Acid Green 5) is an alkaline stain used for staining animal tissues and for cytoplasmic and fiber staining in plant histology.

1. Masson trichrome staining method
Used to distinguish muscle fibers, collagen fibers and fibrin in tissue sections.
Experimental steps:
1. Fixation: Fix tissue samples with Bouin solution to enhance staining effect.
2. Staining:
1) Use Weigert iron hematoxylin to stain nuclei.
2) Use Biebrich Scarlet-Acid Fuchsin to stain cytoplasm and muscle fibers.
3) Use phosphomolybdic acid-phosphotungstic acid for differentiation.
4) Use Light Green SF Yellowish to stain collagen fibers.
4) Dehydration and sealing: Dehydrate through gradient alcohol, make xylene transparent, and finally seal with resin medium.
3. Staining results:
1) Collagen fibers: green
2) Muscle fibers: red
30 Cell nuclei: black
2. Papanicolaou staining method
Used to distinguish cells in cytological samples in order to detect abnormalities.
Experimental steps:
1. Fixation: Fix the smear with 95% ethanol.
2. Staining:
Use Harris hematoxylin to stain cell nuclei; use OG-6 (Orange G) to stain keratinocytes; use EA solution (including Eosin Y, Light Green SF Yellowish and Bismarck Brown) to stain cytoplasm.
3. Dehydration and mounting: Dehydrate and transparently mount the slides for microscopic observation.
4. Staining results:
1) Surface cells: stained green or blue-green by Light Green SF Yellowish.
2) Intermediate and parabasal cells: stained pink to red.
3) Cell nuclei: blue.