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Hoechst 33342 analog 2 trihydrochloride

Name: Hoechst 33342 analog 2 trihydrochloride
Brand: TargetMol
SKU: T19012
Rating: 4.5 (1 reviews)

Catalog No. T19012 Copy Product Info

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Hoechst 33342 analog 2 trihydrochloride is a live nuclear marker dye in the Hoechst series. It binds specifically to the minor grooves of DNA (A/T-rich regions) with significant fluorescence enhancement, used for live cell nuclear staining and quantification.

Hoechst 33342 analog 2 trihydrochloride

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Catalog No. T19012

Cas No. 155815-98-8

Pack Size	Price	USA Stock	Global Stock	Quantity
5 mg	$399	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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Custom-made molecule. TargetMol’s expert synthesis team delivers cost-effective solutions. Contact us for inquiries. Committed to serving you.However, due to objective factors, there is a small probability that the synthesis may not be successful during the R&D process. We appreciate your understanding.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	Hoechst 33342 analog 2 trihydrochloride is a live nuclear marker dye in the Hoechst series. It binds specifically to the minor grooves of DNA (A/T-rich regions) with significant fluorescence enhancement, used for live cell nuclear staining and quantification.
In vitro	Hoechst 33342 analog 2 trihydrochloride diluted to a 10 μg/mL working solution can be used for cell staining; it specifically labels nuclear DNA and can be visualized by fluorescence microscopy or flow cytometry [1].

Chemical Properties

Molecular Weight	659.78
Formula	C₂₅H₂₆Cl₃IN₆O
Cas No.	155815-98-8
Smiles	IC1=C(O)C=CC(C2=NC3=C(N2)C=C(C4=NC5=CC=C(N6CCN(CC6)C)C=C5N4)C=C3)=C1.Cl.Cl.Cl
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 3.20 mg/mL (4.85 mM), Sonication is recommmended.

H2O: 16.00 mg/mL (24.25 mM), Sonication is recommmended.

Solution Preparation Table

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	1.5157 mL	7.5783 mL	15.1566 mL	75.7828 mL

H2O

	1mg	5mg	10mg	50mg
5 mM	0.3031 mL	1.5157 mL	3.0313 mL	15.1566 mL
10 mM	0.1516 mL	0.7578 mL	1.5157 mL	7.5783 mL
20 mM	0.0758 mL	0.3789 mL	0.7578 mL	3.7891 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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