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Phloxine B

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Catalog No. T37756Cas No. 18472-87-2

Phloxine B is a red dye, the USP grade of which is used as a color additive for food, drugs, and cosmetics. It has also been used as an alternative to Gram staining, to differentiate between Gram-negative and Gram-positive bacteria. At 100 μg/ml, it can inhibit methicillin-resistant S. aureus growth in vitro. Phloxine B has also been shown to modulate channel activity of wild-type cystic fibrosis transmembrane conductance regulator chloride channels (CFTR; Ki = 38 μM) and mutant delta F508 CFTR (Ki = 33 μM).

Phloxine B

Phloxine B

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Catalog No. T37756Cas No. 18472-87-2
Phloxine B is a red dye, the USP grade of which is used as a color additive for food, drugs, and cosmetics. It has also been used as an alternative to Gram staining, to differentiate between Gram-negative and Gram-positive bacteria. At 100 μg/ml, it can inhibit methicillin-resistant S. aureus growth in vitro. Phloxine B has also been shown to modulate channel activity of wild-type cystic fibrosis transmembrane conductance regulator chloride channels (CFTR; Ki = 38 μM) and mutant delta F508 CFTR (Ki = 33 μM).
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 g$17835 days35 days
25 g$41735 days35 days
50 g$64935 days35 days
100 g$86235 days35 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Phloxine B is a red dye, the USP grade of which is used as a color additive for food, drugs, and cosmetics. It has also been used as an alternative to Gram staining, to differentiate between Gram-negative and Gram-positive bacteria. At 100 μg/ml, it can inhibit methicillin-resistant S. aureus growth in vitro. Phloxine B has also been shown to modulate channel activity of wild-type cystic fibrosis transmembrane conductance regulator chloride channels (CFTR; Ki = 38 μM) and mutant delta F508 CFTR (Ki = 33 μM).
Chemical Properties
Molecular Weight829.63
FormulaC20H2Br4Cl4Na2O5
Cas No.18472-87-2
Smiles[Na].Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C22OC(=O)c3c2c(Cl)c(Cl)c(Cl)c3Cl)c1BrjcExt:v:0:0
Relative Density.0.871 g/cm3 at 20℃
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
Ethanol: 2 mg/mL (2.41 mM), Sonication is recommended.
DMF: 30 mg/mL (36.16 mM), Sonication is recommended.
DMSO: 30 mg/mL (36.16 mM), Sonication is recommended.
PBS (pH 7.2): 10 mg/mL (12.05 mM), Sonication is recommended.
Solution Preparation Table
Ethanol/PBS (pH 7.2)/DMF/DMSO
1mg5mg10mg50mg
1 mM1.2054 mL6.0268 mL12.0536 mL60.2678 mL
PBS (pH 7.2)/DMF/DMSO
1mg5mg10mg50mg
5 mM0.2411 mL1.2054 mL2.4107 mL12.0536 mL
10 mM0.1205 mL0.6027 mL1.2054 mL6.0268 mL
DMF/DMSO
1mg5mg10mg50mg
20 mM0.0603 mL0.3013 mL0.6027 mL3.0134 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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