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2,6-Dichlorophenolindophenol sodium hydrate

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Catalog No. T89068Cas No. 1266615-56-8
Alias DPIP sodium hydrate, DCIP sodium hydrate

2,6-Dichlorophenolindophenol sodium hydrate (DCPIP) is a blue-colored redox dye commonly employed as an indicator for the determination of vitamin C (ascorbic acid) content. DCPIP undergoes a reduction reaction with vitamin C, during which it changes from a blue-colored oxidized state to a colorless reduced form, providing a simple visual assay for ascorbic acid. Beyond vitamin C determination, DCPIP is utilized in diverse research applications, including the evaluation of enzymatic activity of gold nanoparticles, the development of UVB-specific dosimeters, its role as an electron acceptor in histochemical staining techniques, and investigations of mitochondrial electron transport chain activity.

2,6-Dichlorophenolindophenol sodium hydrate

2,6-Dichlorophenolindophenol sodium hydrate

😃Good
Purity: 99.46%
Catalog No. T89068Alias DPIP sodium hydrate, DCIP sodium hydrateCas No. 1266615-56-8
2,6-Dichlorophenolindophenol sodium hydrate (DCPIP) is a blue-colored redox dye commonly employed as an indicator for the determination of vitamin C (ascorbic acid) content. DCPIP undergoes a reduction reaction with vitamin C, during which it changes from a blue-colored oxidized state to a colorless reduced form, providing a simple visual assay for ascorbic acid. Beyond vitamin C determination, DCPIP is utilized in diverse research applications, including the evaluation of enzymatic activity of gold nanoparticles, the development of UVB-specific dosimeters, its role as an electron acceptor in histochemical staining techniques, and investigations of mitochondrial electron transport chain activity.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
100 mg$29-In Stock
500 mg$53-In Stock
1 g$79-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.46%
Appearance:Solid
Color:Purple to Black
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Product Introduction

Bioactivity
Description
2,6-Dichlorophenolindophenol sodium hydrate (DCPIP) is a blue-colored redox dye commonly employed as an indicator for the determination of vitamin C (ascorbic acid) content. DCPIP undergoes a reduction reaction with vitamin C, during which it changes from a blue-colored oxidized state to a colorless reduced form, providing a simple visual assay for ascorbic acid. Beyond vitamin C determination, DCPIP is utilized in diverse research applications, including the evaluation of enzymatic activity of gold nanoparticles, the development of UVB-specific dosimeters, its role as an electron acceptor in histochemical staining techniques, and investigations of mitochondrial electron transport chain activity.
SynonymsDPIP sodium hydrate, DCIP sodium hydrate
Chemical Properties
Molecular Weight308.09
FormulaC12H8Cl2NNaO3
Cas No.1266615-56-8
SmilesO=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)[O-].[Na+].O
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 25 mg/mL (81.15 mM), Sonication is recommended.
H2O: insoluble
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM3.2458 mL16.2290 mL32.4580 mL162.2902 mL
5 mM0.6492 mL3.2458 mL6.4916 mL32.4580 mL
10 mM0.3246 mL1.6229 mL3.2458 mL16.2290 mL
20 mM0.1623 mL0.8115 mL1.6229 mL8.1145 mL
50 mM0.0649 mL0.3246 mL0.6492 mL3.2458 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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