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2,6-Dichlorophenolindophenol sodium hydrate (DCPIP) is a blue-colored redox dye commonly employed as an indicator for the determination of vitamin C (ascorbic acid) content. DCPIP undergoes a reduction reaction with vitamin C, during which it changes from a blue-colored oxidized state to a colorless reduced form, providing a simple visual assay for ascorbic acid. Beyond vitamin C determination, DCPIP is utilized in diverse research applications, including the evaluation of enzymatic activity of gold nanoparticles, the development of UVB-specific dosimeters, its role as an electron acceptor in histochemical staining techniques, and investigations of mitochondrial electron transport chain activity.

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 mg | $29 | - | In Stock | |
| 500 mg | $53 | - | In Stock | |
| 1 g | $79 | - | In Stock |
| Description | 2,6-Dichlorophenolindophenol sodium hydrate (DCPIP) is a blue-colored redox dye commonly employed as an indicator for the determination of vitamin C (ascorbic acid) content. DCPIP undergoes a reduction reaction with vitamin C, during which it changes from a blue-colored oxidized state to a colorless reduced form, providing a simple visual assay for ascorbic acid. Beyond vitamin C determination, DCPIP is utilized in diverse research applications, including the evaluation of enzymatic activity of gold nanoparticles, the development of UVB-specific dosimeters, its role as an electron acceptor in histochemical staining techniques, and investigations of mitochondrial electron transport chain activity. |
| Synonyms | DPIP sodium hydrate, DCIP sodium hydrate |
| Molecular Weight | 308.09 |
| Formula | C12H8Cl2NNaO3 |
| Cas No. | 1266615-56-8 |
| Smiles | O=C1C(Cl)=C/C(C=C1Cl)=N/C2=CC=C(C=C2)[O-].[Na+].O |
| Storage | Keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | ||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 25 mg/mL (81.15 mM), Sonication is recommended. H2O: insoluble | ||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||
Dissolve 2 mg of the compound in 100 μL DMSO
to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO
stock solution to 400 μL PEG300
and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O
and mix thoroughly until a homogeneous solution is obtained.
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