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5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

Name: 5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside
Brand: TargetMol
SKU: T85479
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T85479Cas No. 15548-60-4

Alias X-glucoside, X-Glc

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.

5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside

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Catalog No. T85479Alias X-glucoside, X-GlcCas No. 15548-60-4

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$31	In Stock	In Stock
500 mg	$53	In Stock	In Stock
1 g	$74	In Stock	In Stock
2 g	$108	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Appearance:Solid

Color:White

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COA

Product Introduction

Bioactivity

Description	5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside (X-Glc) is a chromogenic substrate for β-galactosidase, producing a blue product under β-galactosidase catalysis, commonly used for in situ β-galactosidase staining and blue-white screening.
In vitro	5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside is a biochemical reagent used as a biomaterial or organic compound in life sciences research.
Synonyms	X-glucoside, X-Glc

Chemical Properties

Molecular Weight	408.63
Formula	C₁₄H₁₅BrClNO₆
Cas No.	15548-60-4
Smiles	O(C=1C=2C(NC1)=CC=C(Br)C2Cl)[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O

Storage & Solubility Information

Storage

store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 80 mg/mL (195.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (8.08 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4472 mL	12.2360 mL	24.4720 mL	122.3601 mL
5 mM	0.4894 mL	2.4472 mL	4.8944 mL	24.4720 mL
10 mM	0.2447 mL	1.2236 mL	2.4472 mL	12.2360 mL
20 mM	0.1224 mL	0.6118 mL	1.2236 mL	6.1180 mL
50 mM	0.0489 mL	0.2447 mL	0.4894 mL	2.4472 mL
100 mM	0.0245 mL	0.1224 mL	0.2447 mL	1.2236 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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