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Fuchsine base monohydrochloride
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Catalog No. T18994Cas No. 632-99-5
Alias Rosaniline Base monohydrochloride, Magenta base monohydrochloride, Basic Fuchsin monohydrochloride
Fuchsine base monohydrochloride (Rosaniline Base monohydrochloride) is a magenta dye and uses for acid-fast staining with carbol-fuchsin.
Fuchsine base monohydrochloride
😃Good
Catalog No. T18994Alias Rosaniline Base monohydrochloride, Magenta base monohydrochloride, Basic Fuchsin monohydrochlorideCas No. 632-99-5
Fuchsine base monohydrochloride (Rosaniline Base monohydrochloride) is a magenta dye and uses for acid-fast staining with carbol-fuchsin.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 g | $29 | - | In Stock | |
| 10 g | $40 | - | In Stock |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Appearance:Solid
Color:Black
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Fuchsine base monohydrochloride (Rosaniline Base monohydrochloride) is a magenta dye and uses for acid-fast staining with carbol-fuchsin. |
| Cell Research | 1. Carbol Fuchsin Staining Method 1. Prepare the carbol fuchsin solution, which consists of fuchsine and phenol. 2. Drop the dye solution on the sample on the slide. 3. Heat the sample and maintain a certain temperature (such as around 60°C) to allow the dye to penetrate the cell structure. 4. Stain for about 5-10 minutes. 5. Then use acid alcohol (usually 3% hydrochloric acid alcohol) to decolorize and remove unwanted staining. 6. Finally, use contrast staining such as methylene blue or Gram staining for final observation. 2. Acid-fast staining: 1. Because carbol fuchsin has a strong affinity with acid and alcohol, it is often used to stain acid-fast bacteria (such as Mycobacterium tuberculosis). 2. These bacteria can remain red during the staining process, while other bacteria are removed and appear blue or other colors. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | Rosaniline Base monohydrochloride, Magenta base monohydrochloride, Basic Fuchsin monohydrochloride |
| Molecular Weight | 337.85 |
| Formula | C20H20ClN3 |
| Cas No. | 632-99-5 |
| Smiles | NC1=CC=C(/C(C2=CC=C(N)C=C2)=C3C=CC(C=C\3)=N)C=C1C.[H]Cl |
| Relative Density. | 0.999 g/mL at 20°C |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 55 mg/mL (162.79 mM), Sonication is recommended.  | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween-80+45% Saline: 2.5 mg/mL (7.4 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Stock Solution Preparation:
Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
Preparation of the In Vivo Formulation:
1) Add 100 μL of the DMSO stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 μL Tween 80 and mix well until fully clarified.
3) Add 450 μL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
Dose Conversion
You can also refer to dose conversion for different animals. More Dose Conversion
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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