Shopping Cart

Remove All

Your shopping cart is currently empty

Proceed to checkout Continue shopping

Nile Blue A sulfate

Name: Nile Blue A sulfate
Brand: TargetMol
SKU: T19034
Rating: 4.5 (1 reviews)

Copy Product Info

😃Good

Catalog No. T19034Cas No. 3625-57-8

Alias Nile blue sulfate

Nile Blue A (Nile blue sulfate) is employed for differentiating melanins and lipofuscins, staining fats, and preparing amperometric glucose sensors.

Nile Blue A sulfate

Copy Product Info

😃Good

Catalog No. T19034Alias Nile blue sulfateCas No. 3625-57-8

Nile Blue A (Nile blue sulfate) is employed for differentiating melanins and lipofuscins, staining fats, and preparing amperometric glucose sensors.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,520	2-4 weeks	2-4 weeks
50 mg	$1,980	2-4 weeks	2-4 weeks
100 mg	$2,500	2-4 weeks	2-4 weeks

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Resource Download

Product Introduction

Bioactivity

Description	Nile Blue A (Nile blue sulfate) is employed for differentiating melanins and lipofuscins, staining fats, and preparing amperometric glucose sensors.
In vitro	Nile blue A is an effective stain for PHB (polyhydroxybutyrate) granules in bacteria, outperforming Sudan black B in this role. It produces a strong orange fluorescence in poly-p(3-hydroxybutyrate) granules and stains a greater number of PHB granules than Sudan black B, while also being less susceptible to being washed out of the cell during decolorization procedures. Aside from staining polyhydroxyalkanoic acid-accumulating microorganisms or detecting polyhydroxyalkanoic acids in them, Nile blue A can stain Escherichia coli cells lacking detectable polyhydroxyalkanoic acids without interfering with the surface display or specific labeling of peptides. It offers a convenient and cost-effective method for staining E. coli in flow cytometry, serving as an alternative to other fluorescent dyes or intracellular expression markers like green fluorescent protein. Additionally, Nile blue A acts as a powerful photosensitizer in photodynamic therapy, spreading through the body via the bloodstream upon intravenous administration and interacting with various biomolecules in most cells.
Synonyms	Nile blue sulfate

Chemical Properties

Molecular Weight	414.46
Formula	C₂₀H₂₀N₃O₅S
Cas No.	3625-57-8
Smiles	[O-]S([O-])(=O)=O.CCN(CC)c1ccc2nc3c(cc(N)c4ccccc34)[o+]c2c1
Relative Density.	no data available

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 150 mg/mL (361.92 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4128 mL	12.0639 mL	24.1278 mL	120.6389 mL
5 mM	0.4826 mL	2.4128 mL	4.8256 mL	24.1278 mL
10 mM	0.2413 mL	1.2064 mL	2.4128 mL	12.0639 mL
20 mM	0.1206 mL	0.6032 mL	1.2064 mL	6.0319 mL
50 mM	0.0483 mL	0.2413 mL	0.4826 mL	2.4128 mL
100 mM	0.0241 mL	0.1206 mL	0.2413 mL	1.2064 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Nile blue SulfateNile Blue A Sulfate