fluorescent probes

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) is a macrophage inhibitor of macrophage inhibitory factor (MIF) interconjugate isomerase with a Ki of 2.9 μM. 3-Cyano-7-hydroxycoumarin belongs to the group of coumarin derivatives, which can be used as molecular probes and fluorescent dyes.

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

Sulfo-Cy3 NHS ester TEA (146368-16-3 free base) is a sulfonated cyanine-based fluorescent labeling reagent with an excitation wavelength of approximately 550 nm and an emission wavelength of approximately 570 nm. Sulfo-Cy3 NHS ester TEA can be used as an amine-reactive fluorescent probe for labeling biomolecules such as antibodies, proteins, peptides, and nucleic acid probes. Sulfo-Cy3 NHS ester TEA is also suitable for applications including immunofluorescence staining, in situ hybridization, and flow cytometry analysis.

(±)-ANAP is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

(±)-ANAP hydrochloride is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

5-Aminofluorescein hydrochloride (5-AF hydrochloride) is a protein fluorescence labeling reagent known for its outstanding fluorescent properties and is extensively utilized in biomedical research for cell imaging and molecular probes. This compound enables the observation of protein localization and dynamic changes, providing insightful biological understanding. Additionally, 5-Aminofluorescein hydrochloride is employed to detect interactions between biomolecules, aiding in the elucidation of complex biological processes.

WYneC is a chemically selective probe utilized for the detection of thiolated proteins in live cells. This probe is selective for dipeptides that contain thio-amino acids and can be further coupled with azide-containing fluorescent probes for detection purposes.

WYneN is a chemoselective probe designed for the detection of sulfenylated proteins in living cells. In these cells, WYneN binds to sulfenylated proteins and can further interact with azide-containing fluorescent probes for detection purposes.

Fmoc-protected fluorescent amino acid is designed for solid-phase peptide synthesis, specifically for crafting PAINT imaging probes. It features excitation and emission maxima (λ) at 488 nm and 601 nm, respectively.

Sulfo-QSY21-NHS is a fluorescent dye featuring an NHS group that can react with amine groups (-NH2) in proteins or other molecules to form stable amide bonds. It is suitable for synthesizing active group probes.

Pyrromethene 567 is a fluorescent dye (Ex/Em ≈ 518/547 nm) that is commonly used for labeling biomolecules such as proteins, nucleotides, and oligonucleotides. It can also be used to prepare various fluorescent probes or labels, including lipid molecules and microspheres, and is widely applied in bioimaging and molecular detection studies.

Cyanine3 carboxylic acid is a non-toxic red fluorescent dye that serves as a non-reactive fluorophore for experimental controls and calibration. It can also be conjugated with targeting active molecules to create fluorescent probes for rapid detection of molecular reactions. [Ex/Em(nm)=550/570nm]

Cyamine3.5 NHS ester is a squaraine dye utilized in the development of fluorescent probes and biological labeling, characterized by its intermediate wavelength fluorescence emission properties. Specially designed for reactions with amino groups to form stable covalent bonds, it is ideal for labeling biomolecules. Ex/Em(nm)=591/604.

BP Fluor 405 alkyne triTEA is a blue fluorescent dye frequently used in multicolor applications, such as flow cytometry and super-resolution microscopy employing STORM. It is optimally excited by the 407 nm spectral line of a krypton laser or a 408 nm violet laser diode. BP Fluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form stable triazole linkages. For applications sensitive to the presence of copper, copper-free detection probes (BP Fluor 405 DBCO) are also available.

NBD-hydrazine serves as a fluorescent probe that reacts with aldehydes and ketones, exhibiting excitation and emission maxima at 468 and 535 nm, respectively. Additionally, it is utilized as a precursor in creating malondialdehyde-reactive fluorescent probes and as a synthetic intermediate for developing cupric and chromic ion colorimetric sensors.

PerCP Maleimide is a fluorescent dye that reacts with free thiol groups on proteins. PerCP serves as a red fluorescent protein dye for immunostaining, while Maleimide is utilized in creating fluorescent probes, primarily for the specific detection of thiol analytes.

Vari Fluor 350 TSA (200×) (VF 350 Tyramide) is a fluorescent probe within the Vari Fluor TSA series. These fluorescent probes enable the detection method of high-density in situ labeling of target antigens through horseradish peroxidase (HRP). They are widely used in ELISA, IF, and IHC techniques, providing high detection sensitivity.

PbS/CdS QDs (PbS/CdSe QD) serve as fluorescent probes suitable for in vivo imaging within the second near-infrared window.

SERTlight is a fluorescent probe that specifically labels the soma, dendrites and axonal projection structures of serotonergic neurons corresponding to serotonin transporter (SERT) fluorescent substrates. It is compatible with multiple genetically encoded probes in optical properties, pharmacological effects and experimental operations, enabling multi-channel combined imaging. With SERTlight, GRAB₅HT probes can be used to label distal 5-hydroxytryptamine axonal projections and image endogenous 5-hydroxytryptamine release simultaneously, providing a novel multifunctional molecular tool for the research of the serotonergic nervous system.

Vari Fluor 555-Streptavidin is a dye from the Vari Fluor series, designed by labeling streptavidin with fluorescent probes. Streptavidin is a tetrameric protein with high affinity, comprised of four identical subunits, and it can specifically bind to biotin through reversible non-covalent interactions. This property allows streptavidin to enable the rapid and efficient detection of biotin-labeled molecules, making it a valuable tool in experiments such as immunofluorescence (IF), enzyme-linked immunosorbent assays (ELISA), immunohistochemical staining (IFH), and in situ hybridization. The excitation/emission wavelengths are 555 nm/565 nm.

Sulfo-Cyanine5.5 NHS Ester is a near-infrared fluorescent dye belonging to the cyanine dye family. Its NHS ester group reacts efficiently with primary amines on proteins, antibodies, and other biomolecules under neutral to slightly basic conditions, forming stable amide bonds. The dye exhibits good water solubility and biocompatibility, making it widely used for protein labeling, multicolor fluorescence analysis, and the development of in vivo imaging probes.

BHQ‑3 carboxylic acid is a dark fluorescence quencher containing an azo group. BHQ‑3 carboxylic acid can be conjugated with MMP-cleavable peptides and paired with fluorophores to create activatable probes for in vitro detection of MMP activity. It exhibits peak absorption in the range of 620 to 730 nm. Fluorescent probes based on BHQ‑3 carboxylic acid can be utilized in research on pathological processes related to arthritis and tumors.

Vari Fluor 480 (Vari Fluor 480 TSA 200×) EM is a fluorescent probe part of the Vari Fluor TSA series (Ex/em: 450/480 nm). These probes are designed for high-density in situ labeling of target antigens using the horseradish peroxidase (HRP) detection method, widely utilized in techniques such as ELISA, immunofluorescence, and immunohistochemistry, offering excellent detection sensitivity.