fluorescent probes

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) is a macrophage inhibitor of macrophage inhibitory factor (MIF) interconjugate isomerase with a Ki of 2.9 μM. 3-Cyano-7-hydroxycoumarin belongs to the group of coumarin derivatives, which can be used as molecular probes and fluorescent dyes.

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

(±)-ANAP is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

(±)-ANAP hydrochloride is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

5-Aminofluorescein hydrochloride (5-AF hydrochloride) is a protein fluorescence labeling reagent known for its outstanding fluorescent properties and is extensively utilized in biomedical research for cell imaging and molecular probes. This compound enables the observation of protein localization and dynamic changes, providing insightful biological understanding. Additionally, 5-Aminofluorescein hydrochloride is employed to detect interactions between biomolecules, aiding in the elucidation of complex biological processes.

WYneC is a chemically selective probe utilized for the detection of thiolated proteins in live cells. This probe is selective for dipeptides that contain thio-amino acids and can be further coupled with azide-containing fluorescent probes for detection purposes.

WYneN is a chemoselective probe designed for the detection of sulfenylated proteins in living cells. In these cells, WYneN binds to sulfenylated proteins and can further interact with azide-containing fluorescent probes for detection purposes.

Fmoc-protected fluorescent amino acid is designed for solid-phase peptide synthesis, specifically for crafting PAINT imaging probes. It features excitation and emission maxima (λ) at 488 nm and 601 nm, respectively.

Mgc3Me linker is a connector used for synthesizing mgc3Me fluorescent probes. It modularly attaches the myrGC3Me motif to fluorophores, enabling Golgi apparatus imaging in live cells.

6-(Boc-amino)-1-hexanol is utilized in the synthesis of Hsp90 fluorescent probes and serves as a reagent in the inhibition of deoxyhypusine synthase by GC7.

Sulfo-QSY21-NHS is a fluorescent dye featuring an NHS group that can react with amine groups (-NH2) in proteins or other molecules to form stable amide bonds. It is suitable for synthesizing active group probes.

Cyanine3 carboxylic acid is a non-toxic red fluorescent dye that serves as a non-reactive fluorophore for experimental controls and calibration. It can also be conjugated with targeting active molecules to create fluorescent probes for rapid detection of molecular reactions. [Ex Em(nm)=550 570nm]

Cyamine3.5 NHS ester is a squaraine dye utilized in the development of fluorescent probes and biological labeling, characterized by its intermediate wavelength fluorescence emission properties. Specially designed for reactions with amino groups to form stable covalent bonds, it is ideal for labeling biomolecules. Ex Em(nm)=591 604.

BP Fluor 405 alkyne triTEA is a blue fluorescent dye frequently used in multicolor applications, such as flow cytometry and super-resolution microscopy employing STORM. It is optimally excited by the 407 nm spectral line of a krypton laser or a 408 nm violet laser diode. BP Fluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form stable triazole linkages. For applications sensitive to the presence of copper, copper-free detection probes (BP Fluor 405 DBCO) are also available.

NBD-hydrazine serves as a fluorescent probe that reacts with aldehydes and ketones, exhibiting excitation and emission maxima at 468 and 535 nm, respectively. Additionally, it is utilized as a precursor in creating malondialdehyde-reactive fluorescent probes and as a synthetic intermediate for developing cupric and chromic ion colorimetric sensors.

Quin-2 is a high-affinity fluorescent calcium indicator (Kd = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation emission maxima for quin-2 are 339 and 492 nm, respectively.

ICG-OSu, also known as ICG NHS ester, is a near-infrared fluorescent agent commonly utilized for the development of in vivo imaging probes due to its amine-reactive properties.

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe known for its exceptional sensitivity and selectivity in detecting hypochlorous acid. With an absorption wavelength longer than fluorescein-based probes, it offers improved pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm.

BODIPY-FL (BDP FL acid) is a broad-spectrum and effective fluorescent dye that can be used to label probes or primers and is a compound for the quantitative detection of specific DNA/RNA based on fluorescence bursts.BODIPY-FL-labelled monoterpenes can be used to detect Gram-positive and Gram-negative bacteria as characteristic and pathogenic fungi.

8-Phenyl-BODIPY 505 515 are phenyl-substituted BODIPY derivatives, thereby altering the phosphorescent properties of BODIPY, triplet and monoclinic oxygen generation, and can be used as fluorescent probes.

5-Nitro BAPTA tetramethyl ester, a calcium chelator, is involved in the synthesis of two-photon probes and is used for real-time imaging of intracellular calcium ions and monitoring calcium waves at depths of 100-300 μm in liver tissues for 1100-4000 s. Additionally, in conjunction with fluorescent compound 2-Me-substituted TM, it can form a red fluorescent probe (CaTM-2 AM) [1] [2].

BODIPY R6G methyl ester (compound 5′-1) is a fluorescent dye akin to BODIPY, noted for its excellent photostability and pH insensitivity. This compound is ideal for various research applications, including photodynamic research, PH probes, ion recognition, photocatalysis, protein labeling, cell imaging, and proteomic analysis [1].

PM-1, a Thioflavin-T (ThT) derivative, is a small, highly specific fluorescent dye for plasma membrane (PM) imaging in living and fixed cells. It integrates directly into the cell membrane and retains its presence on the PM for about 15 hours. PM-1 can be combined with protein labeling probes to investigate ectodomain shedding and endocytosis of cell surface proteins [1].