fluorescent probes

Fluorescein Sodium (VST-1001) is a phthalic indicator dye. It is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.

3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) is a macrophage inhibitor of macrophage inhibitory factor (MIF) interconjugate isomerase with a Ki of 2.9 μM. 3-Cyano-7-hydroxycoumarin belongs to the group of coumarin derivatives, which can be used as molecular probes and fluorescent dyes.

2-Di-1-ASP is a mono-stryryl dye and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. It has selective for G-quadruplex (G4) and double-stranded DNA.

Sulfo-Cy3 NHS ester TEA (146368-16-3 free base) is a sulfonated cyanine-based fluorescent labeling reagent with an excitation wavelength of approximately 550 nm and an emission wavelength of approximately 570 nm. Sulfo-Cy3 NHS ester TEA can be used as an amine-reactive fluorescent probe for labeling biomolecules such as antibodies, proteins, peptides, and nucleic acid probes. Sulfo-Cy3 NHS ester TEA is also suitable for applications including immunofluorescence staining, in situ hybridization, and flow cytometry analysis.

(±)-ANAP is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

(±)-ANAP hydrochloride is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

5-Aminofluorescein hydrochloride (5-AF hydrochloride) is a protein fluorescence labeling reagent known for its outstanding fluorescent properties and is extensively utilized in biomedical research for cell imaging and molecular probes. This compound enables the observation of protein localization and dynamic changes, providing insightful biological understanding. Additionally, 5-Aminofluorescein hydrochloride is employed to detect interactions between biomolecules, aiding in the elucidation of complex biological processes.

WYneC is a chemically selective probe utilized for the detection of thiolated proteins in live cells. This probe is selective for dipeptides that contain thio-amino acids and can be further coupled with azide-containing fluorescent probes for detection purposes.

WYneN is a chemoselective probe designed for the detection of sulfenylated proteins in living cells. In these cells, WYneN binds to sulfenylated proteins and can further interact with azide-containing fluorescent probes for detection purposes.

Fmoc-protected fluorescent amino acid is designed for solid-phase peptide synthesis, specifically for crafting PAINT imaging probes. It features excitation and emission maxima (λ) at 488 nm and 601 nm, respectively.

Sulfo-QSY21-NHS is a fluorescent dye featuring an NHS group that can react with amine groups (-NH2) in proteins or other molecules to form stable amide bonds. It is suitable for synthesizing active group probes.

Cyanine3 carboxylic acid is a non-toxic red fluorescent dye that serves as a non-reactive fluorophore for experimental controls and calibration. It can also be conjugated with targeting active molecules to create fluorescent probes for rapid detection of molecular reactions. [Ex/Em(nm)=550/570nm]

Cyamine3.5 NHS ester is a squaraine dye utilized in the development of fluorescent probes and biological labeling, characterized by its intermediate wavelength fluorescence emission properties. Specially designed for reactions with amino groups to form stable covalent bonds, it is ideal for labeling biomolecules. Ex/Em(nm)=591/604.

BP Fluor 405 alkyne triTEA is a blue fluorescent dye frequently used in multicolor applications, such as flow cytometry and super-resolution microscopy employing STORM. It is optimally excited by the 407 nm spectral line of a krypton laser or a 408 nm violet laser diode. BP Fluor 405 alkyne triTEA reacts with azides via a copper-catalyzed click reaction (CuAAC) to form stable triazole linkages. For applications sensitive to the presence of copper, copper-free detection probes (BP Fluor 405 DBCO) are also available.

NBD-hydrazine serves as a fluorescent probe that reacts with aldehydes and ketones, exhibiting excitation and emission maxima at 468 and 535 nm, respectively. Additionally, it is utilized as a precursor in creating malondialdehyde-reactive fluorescent probes and as a synthetic intermediate for developing cupric and chromic ion colorimetric sensors.

PerCP Maleimide is a fluorescent dye that reacts with free thiol groups on proteins. PerCP serves as a red fluorescent protein dye for immunostaining, while Maleimide is utilized in creating fluorescent probes, primarily for the specific detection of thiol analytes.

Vari Fluor 350 TSA (200×) (VF 350 Tyramide) is a fluorescent probe within the Vari Fluor TSA series. These fluorescent probes enable the detection method of high-density in situ labeling of target antigens through horseradish peroxidase (HRP). They are widely used in ELISA, IF, and IHC techniques, providing high detection sensitivity.

PbS/CdS QDs (PbS/CdSe QD) serve as fluorescent probes suitable for in vivo imaging within the second near-infrared window.

Vari Fluor 555-Streptavidin is a dye from the Vari Fluor series, designed by labeling streptavidin with fluorescent probes. Streptavidin is a tetrameric protein with high affinity, comprised of four identical subunits, and it can specifically bind to biotin through reversible non-covalent interactions. This property allows streptavidin to enable the rapid and efficient detection of biotin-labeled molecules, making it a valuable tool in experiments such as immunofluorescence (IF), enzyme-linked immunosorbent assays (ELISA), immunohistochemical staining (IFH), and in situ hybridization. The excitation/emission wavelengths are 555 nm/565 nm.

Sulfo-Cyanine5.5 NHS Ester is a near-infrared fluorescent dye belonging to the cyanine dye family. Its NHS ester group reacts efficiently with primary amines on proteins, antibodies, and other biomolecules under neutral to slightly basic conditions, forming stable amide bonds. The dye exhibits good water solubility and biocompatibility, making it widely used for protein labeling, multicolor fluorescence analysis, and the development of in vivo imaging probes.

Quin-2 is a high-affinity fluorescent calcium indicator (Kd = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation/emission maxima for quin-2 are 339 and 492 nm, respectively.

ICG-OSu, also known as ICG NHS ester, is a near-infrared fluorescent dye with amine-reactive functionality that enables efficient covalent conjugation to biomolecules, ICG-OSu is widely utilized for the development of in vivo imaging probes, supporting applications in optical imaging, biodistribution studies, and real-time visualization of biological processes.

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe known for its exceptional sensitivity and selectivity in detecting hypochlorous acid. With an absorption wavelength longer than fluorescein-based probes, it offers improved pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm.

BODIPY-FL (BDP FL acid) is a broad-spectrum and effective fluorescent dye that can be used to label probes or primers and is a compound for the quantitative detection of specific DNA/RNA based on fluorescence bursts.BODIPY-FL-labelled monoterpenes can be used to detect Gram-positive and Gram-negative bacteria as characteristic and pathogenic fungi.