exonuclease

CRT0044876 (7-NO2-ICA) is a potent and selective APE1 inhibitor with IC50 of ~3 μM.

T5 Exonuclease is a DNA exonuclease that exhibits 5′-3′ exonuclease activity on both single-stranded and double-stranded DNA. It also possesses single-strand endonuclease and 5′-flap endonuclease activity.

Exonuclease III is an enzyme that specifically targets double-stranded DNA (dsDNA) and is involved in DNA repair processes. It possesses both endonuclease activity, with apurinic/apyrimidinic (AP) endonuclease function, and 3'→5' exonuclease activity. Exonuclease III can cleave single-stranded DNA at the 5' phosphodiester bonds from the 3' end to the 5' end.

SARS-CoV-2 RdRp-IN-1 (Compound PAP-2) is a potent inhibitor of SARS-CoV-2 RdRp with an EC50 value of 1.11 μM. This compound interacts directly with SARS-CoV-2 RdRp, effectively resisting exonuclease (ExoN) activity, and demonstrates broad-spectrum antiviral activity against coronaviruses.

EXO1-IN-1 (Compound F684) is a potent and selective inhibitor of exonuclease 1 (EXO1) with an IC50 of 15.7 μM. It inhibits DNA end resection, promotes the accumulation of DNA double-strand breaks, and induces S-phase polyadenylation, thereby disrupting DNA repair pathways in homologous recombination-deficient (HRD) cancer cells. This selectivity effectively targets and kills tumor cells with homologous recombination gene defects, such as BRCA1/2 mutations. EXO1-IN-1 holds promise for researching cancers with homologous recombination deficiencies, including BRCA-related tumors.

SARS-CoV-2 nsp14-IN-11 (Compound 13) is an inhibitor of the NSP14 exonuclease, with a pIC50 value of 4.4. It demonstrates potent and complete inhibition of NSP14 exonuclease activity. SARS-CoV-2 nsp14-IN-11 is applicable for research on SARS-CoV-2 infections.

(Z)-Mirin is a potent Mre11–Rad50–Nbs1 (MRN) complex inhibitor, and inhibits Mre11-associated exonuclease activity.

PFM39 is a selective MRE11 nucleic acid exonuclease inhibitor and Mirin analog that prolongs mitosis.PFM39 inhibits HR and alleviates repair defects caused by EXO1/BLM depletion.

Mirin is a Mre11-Rad50-Nbs1 (MRN) complex inhibitor that inhibits MRN-dependent ATM activation without affecting ATM protein kinase activity. It also inhibits Mre11-associated exonuclease activity, thereby disrupting DNA double-strand break repair.Mirin induces cell cycle arrest in G1 phase.

5'-ODMT cEt N-Bz A Phosphoramidite Amidite, a locked nucleic acid (LNA) analogue, exhibits hybridization and mismatch discrimination capabilities similar to LNA, and shows resistance to exonuclease digestion [1].

Carboxypeptidase B, a peptide exonuclease, specifically degrades peptide chains by progressively hydrolyzing from the C-terminal, releasing free amino acids. It acts exclusively on peptide bonds with basic amino acids (such as arginine and lysine) at the C-terminal residues [1] [2].

Oleclumab (MEDI9447) is a human anti-CD73 monoclonal antibody with anticancer activity that inhibits the extracellular enzyme CD73 nucleic acid exonuclease activity, inhibits the adenosine pathway, and can be used for the study of metastatic desmoplasia-resistant prostate cancer.

H3B-968 is a potent inhibitor of Werner syndrome protein (WRN) with an IC50 of approximately 10 nM, targeting its helicase, ATPase, and exonuclease activities. While WRN is known for its synthetic lethal activity in cancer studies, H3B-968 specifically inhibits WRN's helicase function through competitive ATP antagonism [1].

Dresbuxelimab (AK-119) is a humanized monoclonal antibody targeting CD73 that inhibits its exonuclease activity, applicable for COVID-19 research.

Dexamethasone metasulfobenzoate sodium is an inhibitor of the SARS-CoV-2 exonuclease (ExoN), binding to the catalytic site of ExoN.

NSC 617145 (NSC617145) is an inhibitor of WRN helicase that inhibits the ATPase, but not exonuclease, activity of WRN helicase in a concentration-dependent manner.

Endonuclease IV is an apurinic/apyrimidinic (AP) endonuclease that functions as a DNA repair enzyme. It exhibits endonuclease activity at AP sites, possesses 3' phosphodiesterase activity (allowing it to remove various blocking lesions at the 3' end of DNA), and demonstrates endonuclease activity against oxidative DNA damage, as well as 3' to 5' exonuclease activity.

Exo-β-1,4-xylosidase, Bacteroides ovatus (EC.3.2.1.37), is a specialized exonuclease that targets the non-reducing end β-1,4 glycosidic bonds of xylan and xylooligosaccharides. This enzyme is Ca2+-dependent and facilitates the hydrolysis of β-1,4 glycosidic bonds through reversible metal ion binding, thereby degrading xylan to produce xylose. It is applicable in research areas such as lignocellulose bioconversion, bioethanol production, and optimization of xylan saccharification processes.

Exo-β-1,4-xylosidase, derived from Clostridium stercorarium (EC.3.2.1.37), is an exonuclease that specifically targets the β-1,4 glycosidic bonds at the non-reducing ends of xylan and xylooligosaccharides. This enzyme requires Ca2+ ions, facilitating the hydrolysis of β-1,4 glycosidic bonds through reversible metal ion binding, consequently breaking down xylan into xylose. It is utilized in fields such as lignocellulosic bioconversion, bioethanol production, and the optimization of xylan saccharification processes.

DNA polymerase, NeoApollonia Thermophilus, is an enzyme sourced from thermophilic streptococcus, known for its heat-resistant properties, making it suitable for PCR reactions. Additionally, it possesses both 3’→5’ and 5’→3’ exonuclease activities.

Terminal Transferase, Calf (EC 2.7.7.31) is a template-independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl ends of DNA molecules. It can utilize double-stranded or single-stranded DNA with protruding, recessed, or blunt ends as substrates. Terminal Transferase, Calf (EC 2.7.7.31) lacks 5' or 3' exonuclease activity. The presence of Co2+ in the reaction can enhance the efficiency of end addition.